Recombinant Human ICAM-3/CD50 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1681

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Validation Data Gallery

• 

  Purity of Recombinant Human ICAM-3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human ICAM-3 protein Gln30-His485 (Accession# P32942) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 3385
• Accession: P32942
• PredictedSize: 75.7 kDa
• SDS-PAGE: 90-130 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

ICAM3, also known as CD50 or CDW50, is a protein-coding gene in humans. It encodes a member of the intercellular adhesion molecule (ICAM) family, which are type I transmembrane glycoproteins containing immunoglobulin-like C2-type domains. ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages. ICAM3 is expressed with biased expression in bone marrow, spleen, and 12 other tissues. It is found in epithelium, endothelium, leukocytes, fibroblasts, erythrocytes, platelets, and neurons.

References:

1. Fawcett J. et al. (1992). Nature. 360(6403):481-484.
2. Kristóf E. et al. (2013). Apoptosis. 18(10):1235-1251.
3. Shen W. et al. (2018). Cancer Lett. 422:29-43.