Recombinant Human IL-13 protein (mFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

mFc Tag

Activity

not tested

Cat no : Eg1492

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Validation Data Gallery

• 

  Purity of Recombinant Human IL-13 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human IL-13 protein Gly21-Asn132 (Accession# AAK53823) with a mouse IgG Fc tag at the C-terminus.
• GeneID: 3596
• Accession: AAK53823
• PredictedSize: 39.0 kDa
• SDS-PAGE: 40-60 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase up-regulation. It is a mediator of allergic inflammation and different diseases including asthma. IL-13 has effects on immune cells that are similar to those of the closely related cytokine IL-4.

References:

1. Wynn TA. et al.(2003). Annu Rev Immunol. 21: 425-56.
2. Izuhara K. et al. (2006). Curr Med Chem. 13: 2291-8.
3. Corren J. et al. (2015). Curr Allergy Asthma Rep. 13: 415-20.
4. O'Reilly S. et al. (2013). Biofactors. 39: 593-6.