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Recombinant Human CD126/IL-6R alpha protein (His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 6-23 ng/mL

Cat no : Eg0490

Validation Data Gallery

  • Purity of Recombinant Human IL-6R alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IL-6R alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human IL-6 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human IL-6R alpha (His tag) with a linear range of 6-23 ng/mL.

    Immobilized Human IL-6 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human IL-6R alpha (His tag) with a linear range of 6-23 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human IL-6 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human IL-6R alpha (His tag) with a linear range of 6-23 ng/mL.
Expression HEK293-derived Human IL-6R alpha protein Leu20-Pro365 (Accession# P08887-1) with His tag at the C-terminus.
GeneID 3570
Accession P08887-1
PredictedSize 42.3 kDa
SDS-PAGE 55-80 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin 6 receptor (IL6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines.

References:

1.Arnold P., et al. (2020). Cells. 24;9(5):1307.
2.Yamasaki K., et al. (2020). Neuropsychopharmacol Rep. 40(2):130-137.
3.Hoek KS., et al. (2008). Pigment Cell Melanoma Res. 21(6):665-76.