Recombinant Human IL15RA protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90%, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3588

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Validation Data Gallery

• 

  Purity of Recombinant Human IL15RA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human IL15RA was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90%, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human IL15RA protein Ile31-Thr205 (Accession# Q13261-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 3601
• Accession: Q13261-1
• PredictedSize: 44.7 kDa
• SDS-PAGE: 65-75 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-15 receptor α (IL15RA), also known as CD215 or IL-15Rα, is a member of the type I cytokine receptor family and is an important component of proinflammatory signaling for interleukin 15 (IL15). IL15 and IL15RA are present in the circulation and have been detected in a variety of tissues, where they affect physiological functions such as muscle contractility and overall metabolism. (PMID: 28602725) IL-15Rα signals in both cis and trans, where cis signaling means that IL-15 binds to its receptor on the same cell, and trans signaling means that IL-15 signals through extracellular IL-15Rα. IL15/IL15RA autocrine growth-stimulating loops are found in human T-cell lymphotropic virus 1 ( HTLV-1)-associated adult T-cell leukemia/lymphoma progression. (PMID: 36009415)

References:

1. Loro E, et al. (2017). Bone. 103:20-30.                                                                      
2. Marra P, et al. (2014). Cancer Res. 74(17):4908-21.                                                  
3. Bellón T, et al. (2022). Biomedicines. 10(8):1868.