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Recombinant Human IL-1R2 protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0118

Validation Data Gallery

  • Purity of Recombinant Human IL-1R2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IL-1R2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human IL-1R2 protein Phe14-Glu343 (Accession# P27930-1) with a Myc tag and a His tag at the C-terminus.
GeneID 7850
Accession P27930-1
PredictedSize 42.8 kDa
SDS-PAGE 50-70 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-1 receptor type 2 (IL-1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. IL-1R2 binds interleukin alpha (IL-1A), interleukin beta (IL-1B), and interleukin 1 receptor, type I (IL-1R1/IL-1RA), and acts as a decoy receptor that inhibits the activity of its ligands. It exists in both a membrane bound and soluble form. Soluble molecules are found in cell culture supernatant and in biological fluids under several physiopathological conditions.

References:

1. McMahan C J. et al. The EMBO journal, 1991, 10(10): 2821-2832.
2. Colotta F. et al. Science, 1993, 261(5120): 472-475.
3. Giri J G. et al. The Journal of Immunology, 1994, 153(12): 5802-5809.
4. Neumann D. et al. The Journal of Immunology, 2000, 165(6): 3350-3357.