Human IL-5 ELISA Kit

Sensitivity

30 pg/mL

Range

31.25-2000 pg/mL

Reacted Species

Human

Cat no : KE00010

Synonyms

EDF, IL 5, IL5, IL-5, Interleukin 5, T cell replacing factor, TRF



Product Information

KE000010 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL5 ELISA kit is to be used to detect and quantify protein levels of endogenous IL5. The assay recognizes human IL5. A polyclonal antibody specific for IL5 has been pre-coated onto the microwells. The IL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL5 is added to detect the captured IL5 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Product name Human IL-5 ELISA Kit
Tests 1 X 96 well plate
Sample type Plasma, Serum, Cell culture supernatant, Urine
Assay type Sandwich
Sensitivity 30 pg/mL
Range 31.25-2000 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Plasma97%87-116%
Cell culture supernatant102%91-116%
Urine98%82-123%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

InterAssay

Samplenmean ( pg/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

Background Information

KE000010 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL5 ELISA kit is to be used to detect and quantify protein levels of endogenous IL5. The assay recognizes human IL5. A polyclonal antibody specific for IL5 has been pre-coated onto the microwells. The IL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL5 is added to detect the captured IL5 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms EDF, IL 5, IL5, IL-5, Interleukin 5, T cell replacing factor, TRF