Human IL-9 ELISA Kit
Sensitivity
0.3 pg/mL
Range
3.13-200 pg/mL
Reactivity
Human
Cat no : KE00013
Synonyms
Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40
Validation Data Gallery
Product Information
KE00013 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL9 ELISA kit is to be used to detect and quantify protein levels of endogenous IL9. The assay recognizes human IL9. A polyclonal antibody specific for IL9 has been pre-coated onto the microwells. The IL9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL9 is added to detect the captured IL9 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
Product name | Human IL-9 ELISA Kit |
Tests | 1 X 96 well plate |
Sample type | Serum, Plasma, Cell culture supernatants |
Assay type | Sandwich |
Sensitivity | 0.3 pg/mL |
Range | 3.13-200 pg/mL |
Reactivity | Human |
Tested applications | Sandwich ELISA |
Gene ID (NCBI) | 3578 |
Recovery
Sample Type | Average | Range |
---|---|---|
Plasma | 99% | 86%-112% |
Cell culture supernatant | 103% | 79%-120% |
IntraAssay
Sample | n | mean ( pg/mL) | SD | CV% |
---|---|---|---|---|
1 | 20 | ? ug/ml | ? | ?% |
2 | 20 | ? ug/ml | ? | ?% |
3 | 20 | ? ug/ml | ? | ?% |
InterAssay
Sample | n | mean ( pg/mL) | SD | CV% |
---|---|---|---|---|
1 | 20 | ? ug/ml | ? | ?% |
2 | 20 | ? ug/ml | ? | ?% |
3 | 20 | ? ug/ml | ? | ?% |
Background Information
Interleukin 9 (IL-9) is a γc-family cytokine that is highly produced by T-helper 9 (Th9) cells. IL-9 could promote the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes.
Properties
Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Synonyms | Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40 |