Human IL-9 ELISA Kit

Sensitivity

0.3 pg/mL

Range

3.13-200 pg/mL

Reacted Species

Human

Cat no : KE00013

Synonyms

Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40



Product Information

KE00013 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL9 ELISA kit is to be used to detect and quantify protein levels of endogenous IL9. The assay recognizes human IL9. A polyclonal antibody specific for IL9 has been pre-coated onto the microwells. The IL9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL9 is added to detect the captured IL9 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Product name Human IL-9 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.3 pg/mL
Range 3.13-200 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Plasma99%86%-112%
Cell culture supernatant103%79%-120%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

InterAssay

Samplenmean ( pg/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

Background Information

Interleukin 9 (IL-9) is a γc-family cytokine that is highly produced by T-helper 9 (Th9) cells. IL-9 could promote the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Cytokine P40, HP40, IL 9, IL9, IL-9, interleukin 9, P40, T cell growth factor P40