Recombinant Human KIR2DL2 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1695

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Validation Data Gallery

• 

  Purity of Recombinant Human KIR2DL2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human KIR2DL2 protein His22-His245 (Accession# P43627) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 3803
• Accession: P43627
• PredictedSize: 50.9 kDa
• SDS-PAGE: 55-75 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

KIR2DL2 is one of killer cell immunoglobulin-like receptors, which are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response (PMID: 36719466; 22022261; 9577090).

References:

1. Mora-Bitria L. et al. (2023). Immunogenetics. 75(3):269-282.
2. Seich Al Basatena NK. et al. (2011). PLoS Pathog. 7(10):e1002270.
3. Mingari MC. et al. (1998). Immunol Today. 19(4):153-157.