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Recombinant Human L1CAM protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0209

Validation Data Gallery

  • Purity of Recombinant Human L1CAM was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human L1CAM was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human L1CAM protein Ile20-Glu1120 (Accession# P32004-1) with a Myc tag and a His tag at the C-terminus.
GeneID 3897
Accession P32004-1
PredictedSize 128.2 kDa
SDS-PAGE 120-200 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail. L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts. L1CAM is overexpressed in many human cancers and is often associated with bad prognosis.

References:

1. M Moos. et al. (1988) Nature. 334(6184):701-3.
2. S Kenwrick. et al. (2000) Hum Mol Genet. 9(6):879-86.
3. Helena Kiefel. et al. (2012) Cell Adh Migr. 6(4):374-84.
4. Timur R Samatov. et al. (2016) Prog Histochem Cytochem. 51(2):25-32. 
5. Peter Altevogt. et al. (2016) Int J Cancer. 138(7):1565-76.