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Recombinant Human LEP protein (hIGg2fc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

hIGg2fc Tag

Activity

not tested

Cat no : Eg0328

Validation Data Gallery

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <1.0 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human LEP protein Val22-Cys167 (Accession# P41159) with a hIGg2fc Tag at the N-terminus.
GeneID 3952
Accession P41159
PredictedSize 43.1 kDa
SDS-PAGE
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Leptin is a protein hormone predominantly produced by adipocytes, with key roles in regulating appetite, energy balance, and neuroendocrine function. It signals to the brain, particularly the hypothalamus, to control feeding behavior and energy expenditure. Leptin levels rise after eating, indicating high energy reserves and reducing appetite, while they drop during fasting, stimulating hunger. Additionally, Leptin influences immune function by modulating inflammatory cytokine production and contributing to metabolic homeostasis. Abnormalities in Leptin levels are associated with conditions such as obesity, malnutrition, cardiovascular events, and infertility.

References:

1. Obradovic, Milan et al. Frontiers in endocrinology vol. 12 585887. 18 May. 2021.                                                            2. Pereira, Sandra et al. Endocrine reviews vol. 42,1 (2021): 1-28.                                                                                              3. Perakakis, Nikolaos et al. Journal of the American College of Cardiology vol. 77,6 (2021): 745-760.                                    4. Picó, Catalina, and Mariona Palou. Nutrients vol. 14,1 114. 28 Dec. 2021.