Recombinant Human Lysozyme protein (rFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg4630

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Validation Data Gallery

• 

  Purity of Recombinant Human Lysozyme was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human Lysozyme was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Lysozyme protein Lys19-Val148 (Accession# P61626) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 4069
• Accession: P61626
• PredictedSize: 41.9 kDa
• SDS-PAGE: 40-47 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

LYZ(Lysozyme) is also named as LZM and belongs to the glycosyl hydrolase 22 family, and is a well-known antimicrobial peptide that exists widely in mammalian skin. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. Lysozyme (LYZ) is an integral component of the innate immune system and is known for its antibacterial properties. It is produced by various cell types, with higher expression observed in mucus and saliva.

References:

1. Su Y. et al. (2015) Acta Histochem. 117(8):798-802.
2. Rouchon CN. et al. (2022) Antimicrob Agents Chemother. 66(5):e0233921.
3. Ren Y. et al. (2023) Arch Med Sci. 20(1):233–247.
4. Swaminathan R. et al. (2011) Adv Protein Chem Struct Biol. 84:63-111.
5. Liu J. et al. (2011) Poult Sci. 97(11):3992-3999.