Recombinant Human Meprin beta Subunit/MEP1B protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2875

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Validation Data Gallery

• 

  Purity of Recombinant Human Meprin beta Subunit was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Meprin beta Subunit protein Thr23-Thr652 (Accession# Q16820) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 4225
• Accession: Q16820
• PredictedSize: 97.5 kDa
• SDS-PAGE: 92-130 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Meprin A subunit beta (MEP1B) is a part of the 'astacin family' and 'metzincin superfamily' of metalloendopeptidases. It is a highly glycosylated, disulfide-linked oligomeric protease composed of one or two evolutionarily related subunits, α and β. MEP1B is implicated in the processing of inflammatory mediators, including cytokines and chemokines, which provides a link to its involvement in inflammatory disorders. It also has been associated with several diseases, particularly those involving barrier breakdown such as Alzheimer's disease, kidney disruption, and inflammatory bowel disease.

References:

1.Herzog C. et al. (2019) Cytokine. 114:18-25.
2.Jiang W. et al. (2000) Gene. 248(1-2):77-87.
3.Gindorf M. et al. (2021) J Cereb Blood Flow Metab. 41(1):31-44.