Speedy™ Human/Mouse/Rat Synaptotagmin-1 One-Step ELISA Kit
Cat no : SE50189
Synonyms
Synaptotagmin 1, SYT1, P65, SVP65, synaptotagmin I
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Mouse/Rat Synaptotagmin-1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 16.7 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6857 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 94% | 76%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,620.3 | 41.5 | 2.6 |
| 2 | 8 | 427.7 | 10.7 | 2.5 |
| 3 | 8 | 233.0 | 9.4 | 4.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,648.4 | 67.2 | 4.1 |
| 2 | 16 | 442.3 | 28.0 | 6.3 |
| 3 | 16 | 237.7 | 14.6 | 6.1 |
Background Information
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Synaptotagmin-1 acts as the primary calcium sensor for fast synchronous neurotransmitter release. It contains two C2 domains (C2A and C2B) that mediate Ca²⁺-dependent membrane interactions. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse. In addition to regulating exocytosis, synaptotagmin-1 has also been implicated in endocytosis and neurite outgrowth.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Synaptotagmin 1, SYT1, P65, SVP65, synaptotagmin I |