Human/Mouse/Rat TH ELISA Kit
Cat no : KE00991
Synonyms
tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH
Validation Data Gallery
Product Information
KE00991 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat TH ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat TH. The assay recognizes human/mouse/rat TH. An antibody specific for human/mouse/rat TH has been pre-coated onto the microwells. The human/mouse/rat TH protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat TH is added to detect the captured human/mouse/rat TH protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat TH ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 10.8 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7054 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 97% | 84%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,225.6 | 58.9 | 4.8 |
| 2 | 8 | 312.3 | 20.1 | 6.4 |
| 3 | 8 | 172.7 | 5.1 | 3.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,243.1 | 74.2 | 6.0 |
| 2 | 16 | 305.7 | 17.1 | 5.6 |
| 3 | 16 | 171.9 | 9.2 | 5.4 |
Background Information
Human TH is encoded by the single-copy TH gene, which is located on chromosome 11p15.5 and has 13 exons. This enzyme is present mainly in the central nervous system and adrenal medulla, and its activity is regulated tightly by multiple levels of control, such as modulation of gene expression through alternative splicing of pre-mRNA, post-transcriptional modification of mRNA, and phosphorylation of TH. Traditionally, TH was investigated in the field of neuropsychiatry, because both dopamine and norepinephrine are relevant neurotransmitters synthesized by TH in brain cells. Several studies demonstrated that genetic mutations of TH were important etiologies of nervous system diseases, such as bipolar disorders, schizophrenia, and Parkinson disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH |