Recombinant Human NKG2A protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2571

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Validation Data Gallery

• 

  Purity of Recombinant Human NKG2A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human NKG2A protein Pro94-Leu233 (Accession# P26715-1) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 3821
• Accession: P26715-1
• PredictedSize: 43.2 kDa
• SDS-PAGE: 50-68 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

NKG2A, also known as CD159A, is a single-pass type II membrane protein with extracellular C-type lectin domain. It belongs to the natural killer cell lectin-like receptor family, which is a group of transmembrane proteins preferentially expressed in NK cells. NKG2A can form a heterodimer with CD94. The NKG2A/CD94 complex is an inhibitory receptor that recognizes HLA-E and is expressed by NK cells and a subset of T cells.

References:

1. Chao Zhang. et al. (2019). Nature Communications. volume 10, Article number: 1507.
2. Brett K. Kaiser. et al. (2008). Proc Natl Acad Sci U S A.105(18):6696-701.