Human NQO1 ELISA Kit
Cat no : KE01045
Synonyms
Azoreductase, DHQU, DIA 4, DIA4, DT diaphorase
Validation Data Gallery
Product Information
KE01045 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NQO1 ELISA kit is to be used to detect and quantify protein levels of endogenous human NQO1. The assay recognizes human NQO1. An antibody specific for human NQO1 has been pre-coated onto the microwells. The human NQO1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NQO1 is added to detect the captured human NQO1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NQO1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.09 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1728 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 99% | 93%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 14.11 | 0.75 | 5.30 |
| 2 | 8 | 3.70 | 0.11 | 2.89 |
| 3 | 8 | 2.10 | 0.05 | 2.45 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 14.38 | 0.93 | 6.47 |
| 2 | 16 | 3.78 | 0.17 | 4.57 |
| 3 | 16 | 2.13 | 0.09 | 4.12 |
Background Information
NAD(P)H quinone oxidoreductase 1 (NQO1) catalyses the two electron reduction of quinones and a wide range of other organic compounds. The resulting abundance of NQO1 expression (up to 200-fold) in cancers and a barely detectable expression in body tissues makes it a selective marker of neoplasms. NQO1 can catalyze the repeated futile redox cycling of certain natural and synthetic quinones to their hydroxyquinones, consuming NADPH and generating rapid bursts of cytotoxic reactive oxygen species (ROS) and H2O2.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Azoreductase, DHQU, DIA 4, DIA4, DT diaphorase |