Recombinant Human Neuropilin-1 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2331

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Validation Data Gallery

• 

  Purity of Recombinant Human Neuropilin-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Neuropilin-1 protein Phe22-Lys644 (Accession# O14786-2) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 8829
• Accession: O14786-2
• PredictedSize: 96.2 kDa
• SDS-PAGE: 90-120 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Neuropilin-1 (NRP1) is a transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 has been demonstrated to play a role in the stability and function of Tregs. It interacts with the ligand Semaphorin-4a (Sema4a) expressed on Tregs to enhance the function and survival of Tregs in tumors, restricting the anti-tumor immune response. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. In summary, NRP1 is a multifunctional receptor protein that is involved in nerve, blood vessel, and tumor development, with implications in immune regulation, tumor growth, and angiogenesis.

References:

1. Gotot J. et al. (2018). Immunol Cell Biol. 96(8):852-862.
2. Yang ZG. et al. (2019). Anat Rec (Hoboken). 302(7):1127-1135.
3. Liu SD. et al. (2020). Chin Med J (Engl). 134(5):508-517.
4. Chaudhary B. et al. (2014). Cancer Immunol Immunother. 63(2):81-99.