Recombinant Human Neurophysin 1 protein (mFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

mFc Tag

Activity

not tested

Cat no : Eg2238

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Validation Data Gallery

• 

  Purity of Recombinant Human OXT was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human OXT was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human OXT protein Ala32-Arg125 (Accession# P01178) with a mouse IgG Fc tag at the N-terminus.
• GeneID: 5020
• Accession: P01178
• PredictedSize: 37.3 kDa
• SDS-PAGE: 33-48 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Oxytocin (OXT), a nine-amino-acid peptide produced in the hypothalamus and released by the posterior pituitary, is well-known for its actions in parturition, lactation, and social behavior. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland and acts by binding to oxytocin receptor (OXTR).

References:

1. Erwei Li , et al. (2024) Nature. 625(7993):175-180.
2. Sébastien Dutertre , et al. (2008) J Biol Chem. 283(11):7100-8.