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Recombinant Human PD-L2/CD273 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0507

Validation Data Gallery

  • Purity of Recombinant Human PD-L2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human PD-L2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human PD-L2 protein Leu20-Pro219 (Accession# Q9BQ51-1) with a His tag at the C-terminus.
GeneID 80380
Accession Q9BQ51-1
PredictedSize 26.4 kDa
SDS-PAGE 34-50 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Programmed cell death ligand 2 (PD-L2, also known as CD273, or B7-DC), is one of the B7 family molecules which are type I transmembrane proteins belonging to the immunoglobulin superfamily. PD-L2 was identified both by its homology to PD-L1 and by analysis of genes differentially expressed in libraries generated from dendritic cells (DC) and activated macrophages. It consists of an extracellular region containing an IgV-like domain and an IgC-like domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is a second ligand for PD-1, a costimulatory molecule that plays an inhibitory role in regulating T-cell activation.

References:

1.S Y Tseng, et al. (2001) J Exp Med. 193(7):839-46.
2.Y Latchman, et al. (2001) Nat Immunol. 2(3):261-8. 
3.Spencer C Liang, et al. (2003) Eur J Immunol. 33(10):2706-16.