Recombinant Human PSCA protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2141

---

Validation Data Gallery

• 

  Purity of Recombinant Human PSCA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human PSCA protein Leu12-Ser86 (Accession# O43653) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 8000
• Accession: O43653
• PredictedSize: 34.5 kDa
• SDS-PAGE: 38-55 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Prostate stem cell antigen (PSCA), composed of 123 amino acid residues, is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein belonging to the Thy-1/Ly-6 family. It is highly expressed in normal prostate and further up-regulated in prostate cancer, as well as non-prostatic malignancies including gastric cancer. PSCA plays a critical role in cell adhesion, proliferation, and survival.

References:

1. R E Reiter, et al. (1998) Proc Natl Acad Sci U S A. 95(4):1735-40.
2. Z Gu, et al. (2000) Oncogene. 19(10):1288-96.
3. Hiromi Sakamoto, et al. (2008) Nat Genet. 40(6):730-40.