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Recombinant Human PTH protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3191

Validation Data Gallery

  • Purity of Recombinant Human PTH was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human PTH was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human PTH was greater than 90% as determined by SEC-HPLC.

    The purity of Human PTH was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human PTH protein Ser32-Gln115 (Accession# P01270) with a rabbit IgG Fc tag at the C-terminus.
GeneID 5741
Accession P01270
PredictedSize 35.7 kDa
SDS-PAGE 37-46 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Parathyroid hormone (PTH), also known as parathormone or parathyrin, is a critical peptide hormone that plays a vital role in calcium homeostasis within the human body. Secreted by the parathyroid glands, which are typically located behind the thyroid gland in the anterior neck, PTH is composed of 84 amino acids and is essential for maintaining serum calcium levels. The regulation of calcium is crucial not only for bone health but also for various physiological processes including muscle contraction, nerve function, and blood coagulation.Excessive secretion of PTH, often leading to hypercalcemia (elevated calcium levels) and associated symptoms such as kidney stones, osteoporosis, and neuropsychiatric disturbances. Conversely, insufficient PTH secretion can lead to hypocalcemia (low calcium levels), which may cause muscle cramps, spasms, and convulsions.

References:

1.Dawale K,Agrawal A. (2022). Cureus. 14 (10):e30251.
2.Tang PK, et al. (2021)Vet J. 275:105719. 
3.Jugade SC,Bhalchim S,Karkhanis A. (2022). Contemp Clin Dent. 13 (4):395-398.