Speedy™ Human/Mouse/Rat S100B One-Step ELISA Kit
Cat no : SE50043
Synonyms
S100 beta, Protein S100 B, Protein S100-B, S100 calcium-binding protein B, S-100 protein beta chain
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Mouse/Rat S100B One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate, Human cerebrospinal fluid (CSF) |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.25-16 ng/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6285 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human cerebrospinal fluid (CSF) | 112% | 109%-115% |
| Tissue lysate | 107% | 104%-110% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.98 | 0.19 | 2.38 |
| 2 | 8 | 2.01 | 0.08 | 3.98 |
| 3 | 8 | 0.61 | 0.04 | 6.56 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.91 | 0.22 | 2.78 |
| 2 | 16 | 2.01 | 0.08 | 3.98 |
| 3 | 16 | 0.60 | 0.04 | 6.67 |
Background Information
S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. S100B is highly expressed in astrocytes in the brain. It is also expressed in the Schwann cells of the peripheral nervous system, and outside the nervous system in melanocytes, adipocytes and chondrocyte. Elevated S100B levels in cerebrospinal fluid and serum reported after head trauma, subarachnoid hemorrhage, and stroke were correlated with the extent of brain damage. An association between elevated serum S100B and the presence of brain metastases has been reported. Increased S100B levels may also be found in patients with cerebral small vessel disease (SVD).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | S100 beta, Protein S100 B, Protein S100-B, S100 calcium-binding protein B, S-100 protein beta chain |