Recombinant Human Siglec-8 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2628

---

Validation Data Gallery

• 

  Purity of Recombinant Human Siglec-8 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Siglec-8 protein Met17-Arg354 (Accession# Q9NYZ4-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 27181
• Accession: Q9NYZ4-1
• PredictedSize: 63.0 kDa
• SDS-PAGE: 65-80 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Siglec-8 is a 431-amino acid protein composed of 3 Ig domains with a 358-amino acid extracellular domain and a 47-amino acid tail. Siglec-8 is a member of the CD33-like subgroup of SIGLECs and is expressed specifically in blood cells namely basophil, mast cells, and eosinophils.

References:

1. H Floyd, et al. (2000) J Biol Chem. 275(2):861-6.
2. K K Kikly, et al. (2000) J Allergy Clin Immunol. 105(6 Pt 1):1093-100.