Recombinant Human SIRP alpha/CD172a protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0625

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Validation Data Gallery

• 

  Purity of Recombinant Human SIRP alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human SIRP alpha protein Glu31-Arg370 (Accession# P78324-1) with a His tag at the C-terminus.
• GeneID: 140885
• Accession: P78324-1
• PredictedSize: 38.1 kDa
• SDS-PAGE: 50-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

SIRP Alpha, also known as CD172a or SHPS-1, is a transmembrane glycoprotein that belongs to the SIRP family. SIRP Alpha is a receptor with immunoreceptor tyrosine-based inhibition motifs in its cytoplasmic domain expressed by myeloid cells, including monocytes, macrophages, granulocytes, and a subset of dendritic cells. After activation of SIRP Alpha, Src homology 2 domain-containing protein tyrosine phosphatase-1 (SHP-1) is recruited and regulates receptor tyrosine kinase coupled signaling, participating in the regulation of phagocytosis and polarization of macrophages. SIRP Alpha binds to CD47, a receptor present on all cells (and frequently overexpressed in cancer cells), and this interaction provides a ‘do-not-eat-me’ signal to prevent phagocytosis.

References:

1. Sarfati M, et al. (2008) Curr Drug Targets. 9(10):842-50.
2. Barclay AN, et al. (2014) Annu Rev Immunol. 32:25-50.
3. de Vos AF, et al. (2020) Nat Immunol. 21(6):601-603.
4. Shen Q, et al. (2022) Front Immunol. 13:865579.