Recombinant Human Surfactant protein D protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4996
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human Surfactant protein D protein Ala21-Phe375 (Accession# P35247) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 6441 |
| Accession | P35247 |
| PredictedSize | 61.5 kDa |
| SDS-PAGE | 65-80 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Surfactant protein-D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain. SP-D is primarily expressed and secreted by type II alveolar cells. It plays a central role in the pulmonary host defence and the modulation of allergic responses.
References:
1. Kishore U, et al. (2006) Mol Immunol. 43(9):1293-1315. 2. van de Wetering JK, et al. (2004) Eur J Biochem. 271(7):1229-1249. 3. Hartl D, et al. (2006) Eur J Clin Invest. 36(6):423-435.