Human SPINT2/HAI-2 ELISA Kit
Cat no : KE00992
Synonyms
SPINT2, HAI2, HAI-2, Kop, Kunitz-type protease inhibitor 2
Validation Data Gallery
Product Information
KE00992 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human SPINT2/HAI-2 ELISA kit is to be used to detect and quantify protein levels of endogenous human SPINT2/HAI-2. The assay recognizes human SPINT2/HAI-2. An antibody specific for human SPINT2/HAI-2 has been pre-coated onto the microwells. The human SPINT2/HAI-2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human SPINT2/HAI-2 is added to detect the captured human SPINT2/HAI-2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SPINT2/HAI-2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.08 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10653 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 86% | 79%-97% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 21.63 | 1.03 | 4.76 |
| 2 | 8 | 5.97 | 0.20 | 3.29 |
| 3 | 8 | 2.82 | 0.09 | 3.04 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 22.94 | 1.73 | 7.52 |
| 2 | 16 | 5.71 | 0.35 | 6.14 |
| 3 | 16 | 2.77 | 0.11 | 4.13 |
Background Information
Serine peptidase inhibitor Kunitz type 2 (SPINT2) is a serine protease inhibitor Kunitz type 2, also known as hepatocyte growth factor (HGF) activator (HGFA) inhibitor type 2 (HAI-2). It displays inhibitory activity in membrane serine proteases, such as matriptase and hepsin, as well as secreted proteases such as HGFA and plasmin. SPINT2 is a tumor suppressor gene that inhibits proteases implicated in cancer progression, like HGFA, hepsin and matriptase. SPINT2 regulates cell proliferation, migration and invasion via a reduction in HGFA, leading to apoptosis and necrosis of uterine leiomyosarcoma cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SPINT2, HAI2, HAI-2, Kop, Kunitz-type protease inhibitor 2 |