Recombinant Human STC1 protein (rFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2922

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Validation Data Gallery

• 

  Purity of Recombinant Human STC1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human STC1 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human STC1 protein Thr18-Ala247 (Accession# P52823-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 6781
• Accession: P52823-1
• PredictedSize: 51.9 kDa
• SDS-PAGE: 55-65 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Stanniocalcin-1(STC1) is a hormone-like glycoprotein that has been shown to regulate calcium and phosphorus homeostasis.Tumorous STC1 could interact with Calreticulin (CRT) to trap CRT in mitochondria area, and then reduces membrane CRT.

References:

1. Yeung BH, et al. Mol. Cell Endocrinol. 2012;349:272–280.
2. Lin H, et al. Cancer Cell. 2021;39:480–493.e486.