Speedy™ Human Serpin F1/PEDF One-Step ELISA Kit
Cat no : SE50196
Synonyms
PEDF, PIG35, Cell proliferation-inducing gene 35 protein, EPC 1, EPC-1
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Serpin F1/PEDF One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5176 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 108% | 95%-131% |
| Cell culture supernatant | 98% | 93%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.86 | 0.08 | 1.65 |
| 2 | 8 | 1.20 | 0.02 | 1.67 |
| 3 | 8 | 0.64 | 0.01 | 1.56 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.02 | 0.18 | 3.59 |
| 2 | 16 | 1.23 | 0.03 | 2.44 |
| 3 | 16 | 0.67 | 0.03 | 4.48 |
Background Information
Serpin F1, also known as Pigment Epithelium-Derived Factor (PEDF) or Cell Proliferation-Inducing Gene 35 Protein (PIG35), is a multifunctional protein that belongs to the serpin family. This family of proteins is characterized by their highly conserved core structure, which is critical for their function as serine protease inhibitors. Serpin F1 is secreted and has been shown to strongly inhibit angiogenesis, making it a potent inhibitor of blood vessel growth. It also has anti-tumor and neurotrophic functions, playing a significant role in various biological processes including cell differentiation, tumor suppression, and neuronal protection. Serpin F1 is expressed in quiescent cells and has been implicated in the regulation of gene expression and cellular proliferation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PEDF, PIG35, Cell proliferation-inducing gene 35 protein, EPC 1, EPC-1 |