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Recombinant Human Serpin F1/PEDF protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1152

Validation Data Gallery

  • Purity of Recombinant Human Serpin F1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Serpin F1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human Serpin F1 protein Gln20-Pro418 (Accession# P36955) with a His tag at the C-terminus.
GeneID 5176
Accession P36955
PredictedSize 45.4 kDa
SDS-PAGE 45-55 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Serpin F1, also known as Pigment Epithelium-Derived Factor (PEDF) or Cell Proliferation-Inducing Gene 35 Protein (PIG35), is a multifunctional protein that belongs to the serpin family. This family of proteins is characterized by their highly conserved core structure, which is critical for their function as serine protease inhibitors. Serpin F1 is secreted and has been shown to strongly inhibit angiogenesis, making it a potent inhibitor of blood vessel growth. It also has anti-tumor and neurotrophic functions, playing a significant role in various biological processes including cell differentiation, tumor suppression, and neuronal protection. Serpin F1 is expressed in quiescent cells and has been implicated in the regulation of gene expression and cellular proliferation.

References:

1. J A Huntington. et al. (2011). J Thromb Haemost. 1:26-34.
2. Law RH. et al. (2006). Genome Biol. 7: 216.
3. Kaslik G. et al. (1997). Biochemistry. 36:5455–5464.
4. Huang X. et al. (2011). J Biol Chem. 286: 8740–8751.