Human TDP-43 ELISA Kit
Cat no : KE00009
Synonyms
ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43
Validation Data Gallery
Product Information
KE00009 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TDP-43 ELISA kit is to be used to detect and quantify protein levels of endogenous TDP-43. The assay recognizes human TDP-43. An antibody specific for TDP-43 has been pre-coated onto the microwells. The TDP-43 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for TDP-43 is added to detect the captured TDP-43 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TDP-43 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human cerebrospinal fluid, Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 0.26 ng/mL |
| Range | 6.25-400 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23435 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| human cerebrospinal fluid | 109% | 95%-128% |
| cell lysates | 95% | 79%-121% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 37.2 | 1.6 | 4.2 |
| 2 | 20 | 73.0 | 2.0 | 2.8 |
| 3 | 20 | 144.9 | 6.5 | 4.5 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 37.5 | 3.1 | 8.4 |
| 2 | 24 | 72.9 | 3.2 | 4.4 |
| 3 | 24 | 153.8 | 7.2 | 4.6 |
Background Information
TARDBP was firstly found as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It was also reported to regulate alternate splicing of the CFTR gene. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP43, is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in Amyotrophic lateral sclerosis (ALS). KE00009 is used to quantify TDP-43 level in vivo and it is suitable for sample of CSF and cell lysates. KE00005 is used to quantify TDP-43 level in vivo and it is suitable for sample of serum or plasma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Analyst Diagnosis of idiopathic amyotrophic lateral sclerosis using Fourier-transform infrared spectroscopic analysis of patient-derived skin. | ||
Adv Sci (Weinh) Quantitative Profiling of Nanoscopic Protein Aggregates Reveals Specific Fingerprint of TDP-43-Positive Assemblies in Motor Neuron Disease. |