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Recombinant Human TMIGD2/CD28H protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2264

Validation Data Gallery

  • Purity of Recombinant Human TMIGD2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human TMIGD2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human TMIGD2 protein Leu23-Gly150 (Accession# Q96BF3-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 126259
Accession Q96BF3-1
PredictedSize 40.3 kDa
SDS-PAGE 46-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade. TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs. The predicted molecular weight of the TMIGD2 protein is 31 kDa. The higher molecular weight of TMIGD2 could be associated with its potential glycosylation at its extracellular region.

References:

1. Nader Rahimi, et al.(2012) Mol Biol Cell. 23(9):1646-56. 
2. Yuwen Zhu, et al.(2013) Nat Commun. 4:2043.
3. Hao Wang, et al,(2024) Nat Commun. 15(1):11
4. Nader Rahimi, et al. ( 2012) Mol Biol Cell. 23(9):1646-56