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Recombinant Human VE-cadherin/CD144 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1200

Validation Data Gallery

  • Purity of Recombinant Human VE-cadherin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human VE-cadherin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human VE-cadherin protein Asp48-Gln599 (Accession# P33151-1) with a His tag at the C-terminus.
GeneID 1003
Accession P33151-1
PredictedSize 63.4 kDa
SDS-PAGE 70-85 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins. VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium. VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium. VE-cadherin has also been shown to be required for angiogenesis.

References:

1. Brasch J. et al. (2011). J Mol Biol. 408(1):57-73.
2. Breier G. et al. (1996). Blood. 87(2):630-41.
3. Wallez Y. et al. (2006). Trends Cardiovasc Med. 16(2):55-9.
4. Vestweber D. (2008). Arterioscler Thromb Vasc Biol. 28(2):223-32.