IBA1 Monoclonal antibody

IBA1 Monoclonal Antibody for IHC, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF, IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

1C6A10

Cat no : 66827-1-Ig

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1



Tested Applications

Positive IHC detected inhuman brain tissue, human gliomas tissue, human liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66827-1-Ig targets IBA1 in WB, IF, IHC, ELISA, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IBA1 fusion protein Ag28236
Full Name allograft inflammatory factor 1
Calculated Molecular Weight 17 kDa
GenBank Accession NumberBC009474
Gene Symbol IBA1
Gene ID (NCBI) 199
RRIDAB_2882170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

Protocols

Product Specific Protocols
IHC protocol for IBA1 antibody 66827-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

Fatal iatrogenic cerebral β-amyloid-related arteritis in a woman treated with lecanemab for Alzheimer's disease

Authors - Elena Solopova
IF

Cell Death Dis

ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis

Authors - Yaxuan Zhang
IF

Oxid Med Cell Longev

Senkyunolide I Protects against Sepsis-Associated Encephalopathy by Attenuating Sleep Deprivation in a Murine Model of Cecal Ligation and Puncture.

Authors - Jian Xie
IF

J Neuroinflammation

Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.

Authors - Shiji Huo
humanIHC,IF

Brain Pathol

Differential perivascular microglial activation in the deep white matter in vascular dementia developed post-stroke.

Authors - Yoshiki Hase
IF

Mol Neurobiol

Hmox1 is Identified as a Ferroptosis Hub Gene and Associated with the M1 Type Microglia/Macrophage Polarization in Spinal Cord Injury: Bioinformatics and Experimental Validation

Authors - Yong Xuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nuc (Verified Customer) (10-24-2023)

There is a clear band at 25 kD which is not the right MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: postmortem human frontal cortex
IBA1 Antibody Western Blot validation (1:1000 dilution) in postmortem human frontal cortex (Cat no:66827-1-Ig)