Filter: AllIHC

IHC staining of human brain using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

human IBA1 Monoclonal antibody, PBS Only

IBA1 antibodies are primarily used as markers for microglia, the resident immune cells of the central nervous system, and for macrophages in peripheral tissues. Proteintech has an extensive validated IBA1 antibodies for IHC, IF-P, IF-Fro, ELISA and more.

Cat No. 66827-1-PBS

Clone No.1C6A10

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, ELISA

Synonyms

AIF1, IBA1, 1C6A10, AIF 1, AIF-1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66827-1-PBS targets human IBA1 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28236 Product name: Recombinant human IBA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-147 aa of BC009474 Sequence: MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP Predict reactive species
Full Name	allograft inflammatory factor 1
Calculated Molecular Weight	17 kDa
GenBank Accession Number	BC009474
Gene Symbol	IBA1
Gene ID (NCBI)	199
ENSEMBL Gene ID	ENSG00000204472
RRID	AB_2882170
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P55008
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.