CL488-conjugated IFIH1 Antibody

IFIH1 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-21775

Synonyms

Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, MDA 5, MDA5, RH116



Tested Applications

Positive IF detected inmouse skeletal muscle tissue
For other applications, we recommend the unconjugated version of this antibody, 21775-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The CL488-21775 immunogen is Fusion Protein CL488-conjugated IFIH1 protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFIH1 fusion protein Ag16467
Full Name interferon induced with helicase C domain 1
Calculated molecular weight 1025aa,117 kDa
Observed molecular weight
GenBank accession number BC111750
Gene symbol IFIH1
Gene ID (NCBI) 64135
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. And this anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated IFIH1 antibody CL488-21775Download protocol
Standard Protocols
Click here to view our Standard Protocols