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  • KD/KO Validated

IFIH1/MDA5 Polyclonal antibody

IFIH1/MDA5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21775-1-AP

Synonyms

Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, IFIH1/MDA5, MDA 5, MDA5, RH116, RLR-2



Tested Applications

Positive WB detected inJurkat cells, Raji cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

21775-1-AP targets IFIH1/MDA5 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFIH1/MDA5 fusion protein Ag16467
Full Name interferon induced with helicase C domain 1
Calculated molecular weight 1025 aa, 117 kDa
Observed molecular weight 117-125 kDa
GenBank accession numberBC111750
Gene symbol IFIH1
Gene ID (NCBI) 64135
RRIDAB_10734593
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).

Protocols

Product Specific Protocols
WB protocol for IFIH1/MDA5 antibody 21775-1-APDownload protocol
IHC protocol for IFIH1/MDA5 antibody 21775-1-APDownload protocol
IF protocol for IFIH1/MDA5 antibody 21775-1-APDownload protocol
IP protocol for IFIH1/MDA5 antibody 21775-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
WB,RIP

Cell Stem Cell

ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress.

Authors - Diana Guallar
humanWB

Nat Commun

MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.

Authors - Peipei Zhou
humanWB

Genes Dev

PMID: 30463905

Authors - Rosa M Rubio
humanWB

Nat Commun

HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction.

Authors - Hisashi Akiyama
humanWB

EMBO J

CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS

Authors - Jia Yi
  • KD Validated