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- KD/KO Validated
IFIH1/MDA5 Polyclonal antibody
IFIH1/MDA5 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, RIP, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21775-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 23 publications below |
IP | See 1 publications below |
RIP | See 1 publications below |
Product Information
The immunogen of 21775-1-AP is IFIH1/MDA5 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IFIH1/MDA5 fusion protein Ag16467 |
Full Name | interferon induced with helicase C domain 1 |
Calculated molecular weight | 1025 aa, 117 kDa |
Observed molecular weight | 117-125 kDa |
GenBank accession number | BC111750 |
Gene symbol | IFIH1 |
Gene ID (NCBI) | 64135 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
IHC protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
IP protocol for IFIH1/MDA5 antibody 21775-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Rep Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation. | ||
bioRxiv CD169-mediated restrictive SARS-CoV-2 infection of macrophages induces pro-inflammatory responses. | ||
Radiat Res Endogenous Retrovirus Activation as a Key Mechanism of Anti-Tumor Immune Response in Radiotherapy. | ||
J Gen Virol Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins. |