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IFIH1/MDA5 Antibody

IFIH1/MDA5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, RIP, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21775-1-AP

Synonyms

Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, MDA 5, MDA5, RH116, RLR-2



Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 21775-1-AP immunogen is Fusion Protein IFIH1/MDA5 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFIH1/MDA5 fusion protein Ag16467
Full Name interferon induced with helicase C domain 1
Calculated molecular weight 1025aa,117 kDa
Observed molecular weight 117-125 kDa
GenBank accession number BC111750
Gene symbol IFIH1
Gene ID (NCBI) 64135
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).

Protocols

Product Specific Protocols
WB protocol for IFIH1/MDA5 antibody 21775-1-APDownload protocol
IHC protocol for IFIH1/MDA5 antibody 21775-1-APDownload protocol
IP protocol for IFIH1/MDA5 antibody 21775-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genes Dev

PMID: 30463905

Authors - Rosa M Rubio
mouseWB,RIP

Cell Stem Cell

ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress.

Authors - Diana Guallar
humanWB

Radiat Res

Endogenous Retrovirus Activation as a Key Mechanism of Anti-Tumor Immune Response in Radiotherapy.

Authors - Andrew K Lee
humanWB

J Gen Virol

Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins.

Authors - Wei Zhang
humanIP

Proteomics

Quantitative Proteomics Identified TTC4 as a TBK1 Interactor and a Positive Regulator of SeV-induced Innate Immunity.

Authors - Jun Shang
mouseWB

Hum Mol Genet

ALS-associated genes in SCA2 mouse spinal cord transcriptomes.

Authors - Daniel R Scoles