- Featured Product
- KD/KO Validated
IFIH1/MDA5 Polyclonal Antibody for IHC, IP, WB, ELISA
Cat no : 21775-1-AP
Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, MDA 5, MDA5, RH116, RLR-2
|Positive WB detected in||Jurkat cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The 21775-1-AP immunogen is Fusion Protein IFIH1/MDA5 protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFIH1/MDA5 fusion protein Ag16467|
|Full Name||interferon induced with helicase C domain 1|
|Calculated molecular weight||1025aa,117 kDa|
|Observed molecular weight||117-125 kDa|
|GenBank accession number||BC111750|
|Gene ID (NCBI)||64135|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).
Cell Stem Cell
ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress.
Endogenous Retrovirus Activation as a Key Mechanism of Anti-Tumor Immune Response in Radiotherapy.
J Gen Virol
Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins.
Quantitative Proteomics Identified TTC4 as a TBK1 Interactor and a Positive Regulator of SeV-induced Innate Immunity.
Hum Mol Genet
ALS-associated genes in SCA2 mouse spinal cord transcriptomes.