IFIH1/MDA5 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFIH1/MDA5 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, RIP, IP, IHC,ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 21775-1-AP

Synonyms

Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, MDA 5, MDA5, RH116, RLR-2

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Validation Data Gallery

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  WB analysis of Jurkat using 21775-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21775-1-AP (IFIH1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21775-1-AP

  WB result of IFIH1 antibody (21775-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFIH1 transfected Jurkat cells.

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  IHC staining of human skeletal muscle using 21775-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21775-1-AP (IFIH1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 21775-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21775-1-AP (IFIH1 antibody) at dilution of 1:200 (under 40x lens).

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  IP experiment of Jurkat using 21775-1-AP

  IP result of anti-IFIH1 (IP:21775-1-AP, 4ug; Detection:21775-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

The immunogen of 21775-1-AP is IFIH1/MDA5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFIH1/MDA5 fusion protein Ag16467
• Full Name: interferon induced with helicase C domain 1
• Calculated molecular weight: 1025 aa, 117 kDa
• Observed molecular weight: 117-125 kDa
• GenBank accession number: BC111750
• Gene symbol: IFIH1
• Gene ID (NCBI): 64135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).

Publications

Species	Application	Title
human	WB	Genes Dev; PMID: 30463905; Authors - Rosa M Rubio
mouse	WB	Cell Rep; Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation.; Authors - Yong Shen
human	WB	Radiat Res; Endogenous Retrovirus Activation as a Key Mechanism of Anti-Tumor Immune Response in Radiotherapy.; Authors - Andrew K Lee
human	WB	J Gen Virol; Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins.; Authors - Wei Zhang
human	IP	Proteomics; Quantitative Proteomics Identified TTC4 as a TBK1 Interactor and a Positive Regulator of SeV-induced Innate Immunity.; Authors - Jun Shang
human	WB	J Neurochem; Ganglioside GD3 regulates dendritic growth in newborn neurons in adult mouse hippocampus via modulation of mitochondrial dynamics.; Authors - Fu-Lei Tang ; KD Validated