IFN-alpha 4/IFNA4 Recombinant monoclonal antibody

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  WB analysis using 87105-1-RR

  Untransfected and human IFN-alpha 4 transfected CHO cells were subjected to SDS PAGE followed by western blot with 87105-1-RR (IFNA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 87105-1-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 87105-1-RR (IFNA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity of 87105-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87105-1-RR against Human IFN-alpha 4/IFNA4 were performed. The affinity constant is 14.2 nM.

IFN-alpha 4/IFNA4 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87105-1-RR

Clone No.250992G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IFNA4, IFN-alpha 4, IFN-alpha-4, IFN-α4 , Interferon alpha 4

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Tested Applications

• Positive WB detected in: Transfected with Human IFNA4 CHO cells, Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87105-1-RR targets IFN-alpha 4/IFNA4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4962

  Product name: Recombinant Human IFNA4 protein (rFc Tag)(HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 24-189 aa of BC113642

  Sequence: CDLPQTHSLGNRRALILLAQMGRISHFSCLKDRHDFGFPEEEFDGHQFQKAQAISVLHEMIQQTFNLFSTEDSSAAWEQSLLEKFSTELYQQLNDLEACVIQEVGVEETPLMNEDSILAVRKYFQRITLYLTEKKYSPCAWEVVRAEIMRSLSFSTNLQKRLRRKD

• Full Name: interferon, alpha 4
• GenBank Accession Number: BC113642
• Gene Symbol: IFNA4
• Gene ID (NCBI): 3441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05014
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFN-alpha 4 (IFN-α4 or IFNA4) belongs to the type I interferon family, a group of cytokines primarily produced in response to viral infections. As a key player in the innate immune response, IFN-α4 provides the body's first line of defense against pathogens by inducing an antiviral state in neighboring cells. Produced primarily by macrophages, IFN-α4 exerts its antiviral effects by binding to the interferon alpha receptor (IFNAR) on cell surfaces, thereby activating downstream immune signaling pathways . This binding triggers the expression of antiviral proteins, such as a protein kinase and an oligoadenylate synthetase, which inhibit viral RNA replication and enhance natural killer cell cytotoxicity against viruses.