Anti-Human IFN-alpha 4/IFNA4 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of Transfected CHO using 98647-1-RR (same clone as 98647-1-PBS)

  1x10^6 CHO Cells or IFN-alpha 4/IFNA4 transfected CHO Cells were intracellularly stained with 0.25 ug Anti-Human IFN-alpha 4/IFNA4 Rabbit RecAb (98647-1-RR, Clone:250992C12) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98647-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected CHO using 98647-1-RR (same clone as 98647-1-PBS)

  1x10^6 CHO cells or IFN-alpha 4/IFNA4 transfected CHO Cells were intracellularly stained with 0.25 ug Anti-Human IFN-alpha 4/IFNA4 Rabbit RecAb (98647-1-RR, Clone: 250992C12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98647-1-PBS in a different storage buffer formulation.

• 

  Affinity of 98647-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98647-1-RR against Human IFN-alpha 4/IFNA4 were performed. The affinity constant is 2.79 nM.

IFN-alpha 4/IFNA4 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98647-1-PBS

Clone No.250992C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

IFNA4, IFN-alpha 4, IFN-alpha-4, IFN-α4 , Interferon alpha 4

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Product Information

98647-1-PBS targets IFN-alpha 4/IFNA4 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4962

  Product name: Recombinant Human IFNA4 protein (rFc Tag)(HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 24-189 aa of BC113642

  Sequence: CDLPQTHSLGNRRALILLAQMGRISHFSCLKDRHDFGFPEEEFDGHQFQKAQAISVLHEMIQQTFNLFSTEDSSAAWEQSLLEKFSTELYQQLNDLEACVIQEVGVEETPLMNEDSILAVRKYFQRITLYLTEKKYSPCAWEVVRAEIMRSLSFSTNLQKRLRRKD

• Full Name: interferon, alpha 4
• GenBank Accession Number: BC113642
• Gene Symbol: IFNA4
• Gene ID (NCBI): 3441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05014
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IFN-alpha 4 (IFN-α4 or IFNA4) belongs to the type I interferon family, a group of cytokines primarily produced in response to viral infections. As a key player in the innate immune response, IFN-α4 provides the body's first line of defense against pathogens by inducing an antiviral state in neighboring cells. Produced primarily by macrophages, IFN-α4 exerts its antiviral effects by binding to the interferon alpha receptor (IFNAR) on cell surfaces, thereby activating downstream immune signaling pathways . This binding triggers the expression of antiviral proteins, such as a protein kinase and an oligoadenylate synthetase, which inhibit viral RNA replication and enhance natural killer cell cytotoxicity against viruses.