IFT140 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFT140 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

37

Cat no : 17460-1-AP

Synonyms

c305C8.4, c380F5.1, gs114, IFT140, KIAA0590, WDTC2

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Validation Data Gallery

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  WB analysis of mouse testis using 17460-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP (IFT140 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 17460-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17460-1-AP (IFT140 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IP experiment of rat testis using 17460-1-AP

  IP Result of anti-IFT140 (IP:17460-1-AP, 4ug; Detection:17460-1-AP 1:300) with rat testis tissue lysate 4400ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue, HepG2 cells, rat testis tissue
• Positive IP detected in: rat testis tissue
• Positive IHC detected in: mouse testis tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 27 publications below
• CoIP: See 1 publications below

Product Information

17460-1-AP targets IFT140 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT140 fusion protein Ag11119
• Full Name: intraflagellar transport 140 homolog (Chlamydomonas)
• Calculated molecular weight: 1462 aa, 165 kDa
• Observed molecular weight: 140 kDa
• GenBank accession number: BC035577
• Gene symbol: IFT140
• Gene ID (NCBI): 9742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

Publications

Species	Application	Title
mouse	IF	PLoS Genet; Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.; Authors - Emily Bowie
mouse	IF	J Histochem Cytochem; The Role of IFT140 in Osteogenesis of Adult Mice Long Bone.; Authors - Dike Tao ; KO Validated
human	IF	Mol Genet Genomic Med; Novel IFT140 variants cause spermatogenic dysfunction in humans.; Authors - Xiong Wang
human	IF	Cilia; Cellular ciliary phenotyping indicates pathogenicity of novel variants in IFT140 and confirms a Mainzer-Saldino syndrome diagnosis.; Authors - Machteld M Oud
human	IF	J Cell Sci; Regulation of ciliary retrograde protein trafficking by Joubert syndrome proteins ARL13B and INPP5E.; Authors - Shohei Nozaki
mouse	IHC	J Dent Res; Essential Role of IFT140 in Promoting Dentinogenesis.; Authors - G Li ; KO Validated