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- KD/KO Validated
IFT140 Polyclonal antibody
IFT140 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (2)
WB, IP, IHC, IF, CoIP, ELISA
Cat no : 17460-1-AP
Validation Data Gallery
|Positive WB detected in||mouse testis tissue, HepG2 cells, rat testis tissue|
|Positive IP detected in||rat testis tissue|
|Positive IHC detected in||mouse testis tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C2C12 cells, hTERT-RPE1 cells, mouse testis tissue|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 18 publications below|
|IHC||See 4 publications below|
|IF||See 49 publications below|
|IP||See 1 publications below|
|CoIP||See 1 publications below|
17460-1-AP targets IFT140 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, canine, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT140 fusion protein Ag11119|
|Full Name||intraflagellar transport 140 homolog (Chlamydomonas)|
|Calculated molecular weight||1462 aa, 165 kDa|
|Observed molecular weight||140 kDa|
|GenBank accession number||BC035577|
|Gene ID (NCBI)||9742|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)
|Product Specific Protocols|
|WB protocol for IFT140 antibody 17460-1-AP||Download protocol|
|IHC protocol for IFT140 antibody 17460-1-AP||Download protocol|
|IF protocol for IFT140 antibody 17460-1-AP||Download protocol|
|IP protocol for IFT140 antibody 17460-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.
Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.
Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.
A distal centriolar protein network controls organelle maturation and asymmetry.
Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling.
IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.