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  • KD/KO Validated

IFT140 Polyclonal antibody

IFT140 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17460-1-AP

Synonyms

c305C8.4, c380F5.1, gs114, IFT140, KIAA0590, WDTC2



Tested Applications

Positive WB detected inmouse testis tissue, HepG2 cells, rat testis tissue
Positive IP detected inrat testis tissue
Positive IHC detected inmouse testis tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17460-1-AP targets IFT140 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT140 fusion protein Ag11119
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Calculated molecular weight 1462 aa, 165 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC035577
Gene symbol IFT140
Gene ID (NCBI) 9742
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

Protocols

Product Specific Protocols
WB protocol for IFT140 antibody 17460-1-APDownload protocol
IHC protocol for IFT140 antibody 17460-1-APDownload protocol
IP protocol for IFT140 antibody 17460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

PLoS Genet

Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.

Authors - Emily Bowie
mouseIF

J Histochem Cytochem

The Role of IFT140 in Osteogenesis of Adult Mice Long Bone.

Authors - Dike Tao
  • KO Validated
humanIF

Mol Genet Genomic Med

Novel IFT140 variants cause spermatogenic dysfunction in humans.

Authors - Xiong Wang
humanIF

Cilia

Cellular ciliary phenotyping indicates pathogenicity of novel variants in IFT140 and confirms a Mainzer-Saldino syndrome diagnosis.

Authors - Machteld M Oud
humanIF

J Cell Sci

Regulation of ciliary retrograde protein trafficking by Joubert syndrome proteins ARL13B and INPP5E.

Authors - Shohei Nozaki
mouseIHC

J Dent Res

Essential Role of IFT140 in Promoting Dentinogenesis.

Authors - G Li
  • KO Validated