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IFT140 Antibody 24 Publications

Rabbit Polyclonal| Catalog number: 17460-1-AP

Featured Product KD/KO validated

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Con: 73 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

IFT140 Antibody IF hTERT-RPE1 cells 17460-1-AP

IF result (enrichment to the base (arrowhead) and tip (asterix) of cilia) of anti-IFT140 (17460-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.

IFT140 Antibody IHC mouse testis tissue 17460-1-AP

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17460-1-AP( IFT140 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IFT140 Antibody WB mouse testis tissue 17460-1-AP

mouse testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP( IFT140 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

IFT140 Antibody IHC mouse testis tissue 17460-1-AP

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17460-1-AP( IFT140 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IFT140 Antibody IP rat testis tissue 17460-1-AP

IP Result of anti-IFT140 (IP:17460-1-AP, 4ug; Detection:17460-1-AP 1:300) with rat testis tissue lysate 4400ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse testis tissue, HepG2 cells, rat testis tissue
Positive IP detected in:
rat testis tissue
Positive IHC detected in:
mouse testis tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
hTERT-RPE1 cells
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IFT140 fusion protein Ag11119
Full name:
intraflagellar transport 140 homolog (Chlamydomonas)
Calculated molecular weight:
1462aa,165 kDa
Observed molecular weight:
140 kDa
GenBank accession number:
BC035577
Gene ID (NCBI):
9742
Gene symbol
IFT140
Synonyms
c305C8.4, c380F5.1, gs114, IFT140, KIAA0590, WDTC2

Background

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)


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