• Featured Product
  • KD/KO Validated

IFT140 Polyclonal antibody

IFT140 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 17460-1-AP


c305C8.4, c380F5.1, gs114, IFT140, KIAA0590, WDTC2

Tested Applications

Positive WB detected inHeLa cells, rat testis tissue, HepG2 cells, mouse testis tissue
Positive IP detected inrat testis tissue
Positive IHC detected inmouse testis tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, hTERT-RPE1 cells, mouse testis tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17460-1-AP targets IFT140 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT140 fusion protein Ag11119
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Calculated Molecular Weight 1462 aa, 165 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC035577
Gene Symbol IFT140
Gene ID (NCBI) 9742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)


Product Specific Protocols
WB protocol for IFT140 antibody 17460-1-APDownload protocol
IHC protocol for IFT140 antibody 17460-1-APDownload protocol
IF protocol for IFT140 antibody 17460-1-APDownload protocol
IP protocol for IFT140 antibody 17460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Genet

Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.

Authors - Carol-Anne Martin


Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Authors - Siew Cheng Phua

Sci Adv

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Authors - Chuan Chen

Nat Commun

A distal centriolar protein network controls organelle maturation and asymmetry.

Authors - Lei Wang

Nat Commun

Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling.

Authors - Linh T Vuong

Nat Commun

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

Authors - Nicolas Taulet