HepG2 cells were subjected to SDS PAGE followed by western blot with 17460-1-AP(IFT140 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

mouse testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP( IFT140 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

rat testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP( IFT140 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

rat testis tissue were subjected to SDS PAGE followed by western blot with 17460-1-AP( IFT140 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP( IFT140 antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 17460-1-AP( IFT140 antibody) at dilution of 1:200 (under 40x lens).

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 17460-1-AP( IFT140 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded mouse testis tissue slide using 17460-1-AP( IFT140 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Result of anti-IFT140 (IP:17460-1-AP, 4ug; Detection:17460-1-AP 1:300) with rat testis tissue lysate 4400ug.

IF result (enrichment to the base (arrowhead) and tip (asterix) of cilia) of anti-IFT140 (17460-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.