|Positive WB detected in||mouse testis tissue, HepG2 cells, rat testis tissue|
|Positive IP detected in||rat testis tissue|
|Positive IHC detected in||mouse testis tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17460-1-AP targets IFT140 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT140 fusion protein Ag11119|
|Full Name||intraflagellar transport 140 homolog (Chlamydomonas)|
|Calculated molecular weight||1462 aa, 165 kDa|
|Observed molecular weight||140 kDa|
|GenBank accession number||BC035577|
|Gene ID (NCBI)||9742|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)
Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.
J Histochem Cytochem
The Role of IFT140 in Osteogenesis of Adult Mice Long Bone.
Mol Genet Genomic Med
Novel IFT140 variants cause spermatogenic dysfunction in humans.
Cellular ciliary phenotyping indicates pathogenicity of novel variants in IFT140 and confirms a Mainzer-Saldino syndrome diagnosis.
J Cell Sci
Regulation of ciliary retrograde protein trafficking by Joubert syndrome proteins ARL13B and INPP5E.
J Dent Res
Essential Role of IFT140 in Promoting Dentinogenesis.