- Featured Product
- KD/KO Validated
CoraLite®594-conjugated IFT140 Polyclonal antibody
IFT140 Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-17460
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL594-17460 targets IFT140 in IF applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IFT140 fusion protein Ag11119 |
Full Name | intraflagellar transport 140 homolog (Chlamydomonas) |
Calculated molecular weight | 1462 aa, 165 kDa |
Observed molecular weight | 140 kDa |
GenBank accession number | BC035577 |
Gene symbol | IFT140 |
Gene ID (NCBI) | 9742 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL594 IFT140 antibody CL594-17460 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |