IFT80 Polyclonal antibody

• 

  WB analysis of NIH/3T3 using 25230-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 25230-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25230-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IFT80 Polyclonal Antibody for WB, ELISA

Cat No. 25230-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Synonyms

ATD2, IFT80, KIAA1374, WDR56

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, mouse brain tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

25230-1-AP targets IFT80 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18650

  Product name: Recombinant human IFT80 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 417-777 aa of BC101494

  Sequence: LNAQTVSLSNDTIAIRDKADEKIIFLFEASTGKPLGDGKFLSHKNEILEIALDQKGLTNDRKIAFIDKNRDLCITSVKRFGKEEQIIKLGTMVHTLAWNDTCNILCGLQDTRFIVWYYPNTVYVDRDILPKTLYERDASEFSKNPHIVSFVGNQVTIRRADGSLVHISITPYPAILHEYVSSSKWEDAVRLCRFVKEQTMWACLAAMAVANRDMTTAEIAYAAIGEIDKVQYINSIKNLPSKESKMAHILLFSGNIQEAEIVLLQAGLVYQAIQININLYNWERALELAVKYKTHVDTVLAYRQKFLETFGKQETNKRYLHYAEGLQIDWEKIKAKIEMEITKEREQSSSSQSSKSIGLKP

• Full Name: intraflagellar transport 80 homolog (Chlamydomonas)
• Calculated Molecular Weight: 777 aa, 88 kDa
• Observed Molecular Weight: 88-90 kDa
• GenBank Accession Number: BC101494
• Gene Symbol: IFT80
• Gene ID (NCBI): 57560
• RRID: AB_2879976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2H3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	EMBO Rep; Male infertility-associated Ccdc108 regulates multiciliogenesis via the intraflagellar transport machinery.; Authors - Huijie Zhao
mouse	WB	Mol Neurobiol; Inpp5e Regulated the Cilium-Related Genes Contributing to the Neural Tube Defects Under 5-Fluorouracil Exposure; Authors - Xiuwei Wang
rat	WB	Cell Physiol Biochem; iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.; Authors - Xiaomei Liu
mouse	WB	Life (Basel); The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study; Authors - Hyeran Helen Jeon
mouse	IF	Cell Insight; Endothelial RGS12 governs angiogenesis in inflammatory arthritis by controlling cilia formation and elongation via MYCBP2 signaling; Authors - Gongsheng Yuan