IFT80 Polyclonal antibody

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• IFT80 Polyclonal antibody (25230-1-AP)

IFT80 Polyclonal Antibody for WB, ELISA

Cat No. 25230-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Synonyms

ATD2, IFT, KIAA1374, WD repeat-containing protein 56, WDR56

• 

  WB analysis of NIH/3T3 using 25230-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 25230-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 25230-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 25230-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse kidney tissue, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25230-1-AP targets IFT80 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18650

  Product name: Recombinant human IFT80 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 417-777 aa of BC101494

  Sequence: LNAQTVSLSNDTIAIRDKADEKIIFLFEASTGKPLGDGKFLSHKNEILEIALDQKGLTNDRKIAFIDKNRDLCITSVKRFGKEEQIIKLGTMVHTLAWNDTCNILCGLQDTRFIVWYYPNTVYVDRDILPKTLYERDASEFSKNPHIVSFVGNQVTIRRADGSLVHISITPYPAILHEYVSSSKWEDAVRLCRFVKEQTMWACLAAMAVANRDMTTAEIAYAAIGEIDKVQYINSIKNLPSKESKMAHILLFSGNIQEAEIVLLQAGLVYQAIQININLYNWERALELAVKYKTHVDTVLAYRQKFLETFGKQETNKRYLHYAEGLQIDWEKIKAKIEMEITKEREQSSSSQSSKSIGLKP

• Full Name: intraflagellar transport 80 homolog (Chlamydomonas)
• Calculated Molecular Weight: 777 aa, 88 kDa
• Observed Molecular Weight: 88-90 kDa
• GenBank Accession Number: BC101494
• Gene Symbol: IFT80
• Gene ID (NCBI): 57560
• RRID: AB_2879976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2H3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFT80 is an intraflagellar transport (IFT) protein that plays a crucial role in the formation and function of primary cilia. IFT proteins are key for cilia formation, and it seems that this organelle has developed into a complex signaling center in mammals, providing important sensory functions. IFT80 is a recently-defined component of IFT complex B, and loss of IFT80 in zebrafish results in cystic kidneys and a reduced number of cilia, whereas silencing IFT80 in Tetrahymena thermophila produces shortened or absent cilia. (PMID: 23333501)

Publications

What published studies show

The IFT80 Polyclonal antibody (Cat# 25230-1-AP) has 6 total citations published in J Adv Res, Cell Insight, EMBO Reports, Molecular Neurobiology, Life (Basel), and Cell Physiology and Biochemistry. Research fields span cilia biology and intraflagellar transport, covering topics such as intervertebral disc degeneration, angiogenesis in inflammatory arthritis, multiciliogenesis in male infertility, neural tube defects, bone remodeling, and renal proteomics.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		J Adv Res; Primary cilia protect against intervertebral disc degeneration and spine scoliosis by regulating Hedgehog-P53-mediated cell apoptosis signaling.; Authors - Xinhua Li
mouse	IF	Cell Insight; Endothelial RGS12 governs angiogenesis in inflammatory arthritis by controlling cilia formation and elongation via MYCBP2 signaling; Authors - Gongsheng Yuan
	WB	EMBO Rep; Male infertility-associated Ccdc108 regulates multiciliogenesis via the intraflagellar transport machinery.; Authors - Huijie Zhao
mouse	WB	Mol Neurobiol; Inpp5e Regulated the Cilium-Related Genes Contributing to the Neural Tube Defects Under 5-Fluorouracil Exposure; Authors - Xiuwei Wang
mouse	WB	Life (Basel); The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study; Authors - Hyeran Helen Jeon
rat	WB	Cell Physiol Biochem; iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.; Authors - Xiaomei Liu