IGF1 Polyclonal antibody

IGF1 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, IF, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 28530-1-AP

Synonyms

IBP1, IGF I, IGF1, IGF1A, IGF1B, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C

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Validation Data Gallery

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  IHC staining of human liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

28530-1-AP targets IGF1 in WB, IHC, IF, Cell treatment, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGF1 fusion protein Ag29197
• Full Name: insulin-like growth factor 1 (somatomedin C)
• Calculated molecular weight: 22 kDa
• GenBank accession number: NM_001111283
• Gene symbol: IGF1
• Gene ID (NCBI): 3479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	IHC	Am J Pathol; Single-Cell RNA-Seq Reveals the Heterogeneity of Cell Communications between Schwann Cells and Fibroblasts within the Microenvironment in Vestibular Schwannoma.; Authors - Maoxiang Xu
human	WB	Cell Death Discov; RCAN1.4 attenuates renal fibrosis through inhibiting calcineurin-mediated nuclear translocation of NFAT2; Authors - Jianjian Zhang
	WB	Biotechnol Appl Biochem; Examination of alternate codon bias solutions for expression and purification of recombinant mechano-growth factor in Escherichia coli.; Authors - Jianguo Feng
rat	WB	Evid Based Complement Alternat Med; A Network Pharmacology Approach for Exploring the Mechanisms of Panax notoginseng Saponins in Ischaemic Stroke.; Authors - Cong Wang
mouse	WB	Mol Med Rep; Hydrogen sulfide is a regulator of mammary gland development in prepubescent female mice.; Authors - Jing Zhang
human	Cell treatment	Ann Hepatol; IGF-1 contributes to liver cancer development in diabetes patients by promoting autophagy.; Authors - Yuying Shan