IGF1 Polyclonal antibody

IGF1 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 28530-1-AP

Synonyms

IBP1, IGF I, IGF1, IGF1A, IGF1B, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C

---

Validation Data Gallery

• 

  IHC staining of human liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below

Product Information

28530-1-AP targets IGF1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGF1 fusion protein Ag29197
• Full Name: insulin-like growth factor 1 (somatomedin C)
• Calculated molecular weight: 22 kDa
• GenBank accession number: NM_001111283
• Gene symbol: IGF1
• Gene ID (NCBI): 3479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
	WB	Biotechnol Appl Biochem; Examination of alternate codon bias solutions for expression and purification of recombinant mechano-growth factor in Escherichia coli.; Authors - Jianguo Feng
rat	WB	Evid Based Complement Alternat Med; A Network Pharmacology Approach for Exploring the Mechanisms of Panax notoginseng Saponins in Ischaemic Stroke.; Authors - Cong Wang
mouse	WB	Mol Med Rep; Hydrogen sulfide is a regulator of mammary gland development in prepubescent female mice.; Authors - Jing Zhang
	WB	Mol Nutr Food Res; Milk Polar Lipids Supplementation to Obese Rats During Pregnancy and Lactation Benefited Skeletal Outcomes of Male Offspring.; Authors - Lihua Han
human	WB	Cancer Lett; Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner.; Authors - Jiao Li