IGF1 Polyclonal antibody

IGF1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IHC, IF, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 28530-1-AP

Synonyms

IBP1, IGF I, IGF1, IGF1A, IGF1B, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C



Tested Applications

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28530-1-AP targets IGF1 in WB, IHC, IF, Cell treatment, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGF1 fusion protein Ag29197
Full Name insulin-like growth factor 1 (somatomedin C)
Calculated molecular weight 22 kDa
GenBank accession numberNM_001111283
Gene symbol IGF1
Gene ID (NCBI) 3479
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF1, also named as IBP1, MGF, IGF-IA, and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but has a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

Protocols

Product Specific Protocols
IHC protocol for IGF1 antibody 28530-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner.

Authors - Jiao Li
human,mouseWB

Metabolism

Arginine reverses growth hormone resistance through the inhibition of toll-like receptor 4-mediated inflammatory pathway.

Authors - Jingren Xu
WB

Mol Nutr Food Res

Milk Polar Lipids Supplementation to Obese Rats During Pregnancy and Lactation Benefited Skeletal Outcomes of Male Offspring.

Authors - Lihua Han
mouseWB

Int J Biol Sci

Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation

Authors - Yin Ni
mouseIF

Biomed Pharmacother

Low-dose X-ray irradiation combined with FAK inhibitors improves the immune microenvironment and confers sensitivity to radiotherapy in pancreatic cancer.

Authors - Huanliang Chen
humanCell treatment

Ann Hepatol

IGF-1 contributes to liver cancer development in diabetes patients by promoting autophagy.

Authors - Yuying Shan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hongxue (Verified Customer) (01-17-2023)

IHC staining for mouse liver, works well.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: mouse liver
IGF1 Antibody Immunohistochemistry validation (1:250 dilution) in mouse liver (Cat no:28530-1-AP)