IGF1 Polyclonal antibody

IGF1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, IF, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

27

Reviews (1)

Cat no : 28530-1-AP

Synonyms

IBP1, IGF I, IGF1, IGF1A, IGF1B, IGFI, Insulin like growth factor I, Mechano growth factor, MGF, Somatomedin C

---

Validation Data Gallery

• 

  IHC staining of human liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28530-1-AP (IGF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive IHC detected in: human liver tissue, mouse kidney tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 18 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

28530-1-AP targets IGF1 in WB, IHC, IF, ELISA, Cell treatment applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGF1 fusion protein Ag29197
• Full Name: insulin-like growth factor 1 (somatomedin C)
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: NM_001111285.2
• Gene Symbol: IGF1
• Gene ID (NCBI): 3479
• RRID: AB_2881164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF1, also named as IBP1, MGF, IGF-IA, and Somatomedin-C, belongs to the INS family. IGF1 is structurally and functionally related to INS but has a much higher growth-promoting activity. Altered expression or mutation of IGF-1 is associated with several human disorders, including type I diabetes and various forms of cancer. Defects in IGF1 are the cause of INS-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

Publications

Species	Application	Title
human	WB	Cancer Lett; Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner.; Authors - Jiao Li
human,mouse	WB	Metabolism; Arginine reverses growth hormone resistance through the inhibition of toll-like receptor 4-mediated inflammatory pathway.; Authors - Jingren Xu
mouse	WB	J Nutr Biochem; Resistant Starch Confers Protection of Dietary Against Diabetic Cardiomyopathy; Authors - Qingqing Zhu
	WB	Mol Nutr Food Res; Milk Polar Lipids Supplementation to Obese Rats During Pregnancy and Lactation Benefited Skeletal Outcomes of Male Offspring.; Authors - Lihua Han
human	WB	J Cell Mol Med; Amentoflavone reverses epithelial-mesenchymal transition in hepatocellular carcinoma cells by targeting p53 signalling pathway axis; Authors - Hui-Ying Jian
mouse	WB	Int J Biol Sci; Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation; Authors - Yin Ni

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Hongxue (Verified Customer) (01-17-2023)

IHC staining for mouse liver, works well.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: mouse liver

IGF1 Antibody Immunohistochemistry validation (1:250 dilution) in mouse liver (Cat no:28530-1-AP)