Validation Data Gallery
|Positive WB detected in||Jurkat cells, rat kidney tissue, HEK-293 cells, HuH-7 cells, mouse kidney tissue, A375 cells, HepG2 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human testis tissue, human kidney tissue, human lung cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A375 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22803-1-AP is IGF2BP1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IGF2BP1 fusion protein Ag18859|
|Full Name||insulin-like growth factor 2 mRNA binding protein 1|
|Calculated molecular weight||577 aa, 63 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC156957|
|Gene ID (NCBI)||10642|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IGF2BP1, also named as CRDBP, VICKZ1, ZBP1, is a 577 amino acid protein, which belongs to the RRM IMP/VICKZ family. IGF2BP1 can form homodimers and heterodimers with IGF2BP1 and IGF2BP3. IGF2BP1 is mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus and also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta. IGF2BP1 is Expressed in various cancers, including testis and lung cancers, as well as kidney, prostate and trachea cancers. IGF2BP1 as RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. The calcualted molecular weight of IGF2BP1 is 63 kDa, but modified IGF2BP1 is about 65-70 kDa.
|Product Specific Protocols|
|WB protocol for IGF2BP1 antibody 22803-1-AP||Download protocol|
|IHC protocol for IGF2BP1 antibody 22803-1-AP||Download protocol|
|IF protocol for IGF2BP1 antibody 22803-1-AP||Download protocol|
|IP protocol for IGF2BP1 antibody 22803-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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