Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, HuH-7 cells, Jurkat cells, mouse kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human lung cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22803-1-AP targets IGF2BP1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18859 Product name: Recombinant human IGF2BP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 126-198 aa of BC156957 Sequence: YSNREQTRQAIMKLNGHQLENHALKVSYIPDEQIAQGPENGRRGGFGSRGQPRQGSPVAAGAPAKQQQVDIPL Predict reactive species |
| Full Name | insulin-like growth factor 2 mRNA binding protein 1 |
| Calculated Molecular Weight | 577 aa, 63 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC156957 |
| Gene Symbol | IGF2BP1 |
| Gene ID (NCBI) | 10642 |
| RRID | AB_2879173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZI8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGF2BP1, also named as CRDBP, VICKZ1, ZBP1, is a 577 amino acid protein, which belongs to the RRM IMP/VICKZ family. IGF2BP1 can form homodimers and heterodimers with IGF2BP1 and IGF2BP3. IGF2BP1 is mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus and also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta. IGF2BP1 is Expressed in various cancers, including testis and lung cancers, as well as kidney, prostate and trachea cancers. IGF2BP1 as RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. The calcualted molecular weight of IGF2BP1 is 63 kDa, but modified IGF2BP1 is about 65-70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IGF2BP1 antibody 22803-1-AP | Download protocol |
| IHC protocol for IGF2BP1 antibody 22803-1-AP | Download protocol |
| IP protocol for IGF2BP1 antibody 22803-1-AP | Download protocol |
| WB protocol for IGF2BP1 antibody 22803-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IGF2BP1 Polyclonal antibody (Cat# 22803-1-AP) has 158 citations. These appear across high-impact journals including Cell, Nature Communications, Nature Genetics, Cell Research, Molecular Cell, Gastroenterology, Cancer Research, Oncogene, Cell Death & Disease, Advanced Science, Science Advances, PNAS, and the Journal of Biological Chemistry. Research fields span m6A RNA modification, cancer biology (hepatocellular, gastric, colorectal, lung, breast cancers), neurology, fibrosis, inflammation, and metabolism, with applications mainly in WB, IF, IHC, RIP, and IP.
| Species | Application | Title |
|---|---|---|
Cell In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.
| ||
Cell Synonymous mutations promote tumorigenesis by disrupting m6A-dependent mRNA metabolism | ||
Mol Cancer METTL3-mediated m(6)A modification of CACNA1E promotes osteosarcoma progression and chemoresistance by enhancing WNT7B-mediated Ca(2+) signaling. | ||
Cell Res Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells. | ||
Gastroenterology ALKBH5 Drives Immune Suppression via targeting AXIN2 to Promote Colorectal Cancer and is a Target for Boosting Immunotherapy
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Reviews
What customers say
Users report strong performance for this IGF2BP1 antibody. Two Western Blot reviews (HeLa and HEK293 cells) highlight clear detection at the expected ~70 kDa band with high sensitivity, including faint bands in IP applications, using a 1/1000 dilution. One Immunofluorescence review (1:50 dilution) notes the antibody works but detects signal in the nucleus. Overall, the antibody is well-received for WB with consistent, sensitive results, while IF users should be aware of nuclear localization of the signal.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-13-2024) | Worked very well to detect IGF2BP1 at around 70 kDa by WB in HeLa cells (with an overnight incubation at 4°C, 1/1000 dilution)
|
tao (Verified Customer) (12-14-2022) | Immunofluorescence seems to work, but signal can be detected in the nucleus
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Parisa (Verified Customer) (06-13-2022) | I used the antibody and it worked very well, It was sensitive and could detect the faint band that I expected in my IP.
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