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  • KD/KO Validated

IGF2BP1 Polyclonal antibody

IGF2BP1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22803-1-AP

Synonyms

CRD BP, CRDBP, IGF II mRNA binding protein 1, IGF2 mRNA binding protein 1, IGF2BP1, IMP 1, IMP1, VICKZ family member 1, VICKZ1, ZBP 1, ZBP1, Zip code binding protein 1, Zipcode binding protein 1



Tested Applications

Positive WB detected inJurkat cells, rat kidney tissue, HEK-293 cells, HuH-7 cells, mouse kidney tissue, A375 cells, HepG2 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22803-1-AP is IGF2BP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGF2BP1 fusion protein Ag18859
Full Name insulin-like growth factor 2 mRNA binding protein 1
Calculated molecular weight 577 aa, 63 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC156957
Gene symbol IGF2BP1
Gene ID (NCBI) 10642
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGF2BP1, also named as CRDBP, VICKZ1, ZBP1, is a 577 amino acid protein, which belongs to the RRM IMP/VICKZ family. IGF2BP1 can form homodimers and heterodimers with IGF2BP1 and IGF2BP3. IGF2BP1 is mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus and also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta. IGF2BP1 is Expressed in various cancers, including testis and lung cancers, as well as kidney, prostate and trachea cancers. IGF2BP1 as RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. The calcualted molecular weight of IGF2BP1 is 63 kDa, but modified IGF2BP1 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for IGF2BP1 antibody 22803-1-APDownload protocol
IHC protocol for IGF2BP1 antibody 22803-1-APDownload protocol
IF protocol for IGF2BP1 antibody 22803-1-APDownload protocol
IP protocol for IGF2BP1 antibody 22803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cancer Lett

A novel, liver-specific long noncoding RNA LINC01093 suppresses HCC progression by interaction with IGF2BP1 to facilitate decay of GLI1 mRNA.

Authors - Jia He
humanWB

J Exp Clin Cancer Res

PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α.

Authors - Pingfu Hou
humanWB

J Cell Mol Med

Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.

Authors - Lin-Jian Wang
  • KD Validated
humanWB,RIP

Cell Death Dis

EGR2-mediated regulation of m6A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization.

Authors - Yufan Ying
  • KO Validated
ratWB,IHC

J Cell Mol Med

mTOR-autophagy promotes pulmonary senescence through IMP1 in chronic toxicity of methamphetamine.

Authors - Mei-Jia Zhu
humanWB, IHC

J Biol Chem

MiRNA-873 Inhibits Glioblastoma Tumorigenesis and Metastasis by Suppressing the Expression of IGF2BP1.

Authors - Ren-Jie Wang