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IGF2BP1 Polyclonal antibody
IGF2BP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 22803-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, Jurkat cells, mouse kidney tissue, A375 cells, rat kidney tissue, HepG2 cells, HuH-7 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human lung cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A375 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
22803-1-AP targets IGF2BP1 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IGF2BP1 fusion protein Ag18859|
|Full Name||insulin-like growth factor 2 mRNA binding protein 1|
|Calculated molecular weight||577 aa, 63 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC156957|
|Gene ID (NCBI)||10642|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IGF2BP1, also named as CRDBP, VICKZ1, ZBP1, is a 577 amino acid protein, which belongs to the RRM IMP/VICKZ family. IGF2BP1 can form homodimers and heterodimers with IGF2BP1 and IGF2BP3. IGF2BP1 is mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus and also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta. IGF2BP1 is Expressed in various cancers, including testis and lung cancers, as well as kidney, prostate and trachea cancers. IGF2BP1 as RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. The calcualted molecular weight of IGF2BP1 is 63 kDa, but modified IGF2BP1 is about 65-70 kDa.
|Product Specific Protocols|
|WB protocol for IGF2BP1 antibody 22803-1-AP||Download protocol|
|IHC protocol for IGF2BP1 antibody 22803-1-AP||Download protocol|
|IF protocol for IGF2BP1 antibody 22803-1-AP||Download protocol|
|IP protocol for IGF2BP1 antibody 22803-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
tao (Verified Customer) (12-14-2022)
Immunofluorescence seems to work, but signal can be detected in the nucleus
Parisa (Verified Customer) (06-13-2022)
I used the antibody and it worked very well, It was sensitive and could detect the faint band that I expected in my IP.