IGF2BP1 Polyclonal antibody

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  WB analysis of Jurkat using 22803-1-AP

  WB result of IGF2BP1 antibody (22803-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IGF2BP1 transfected Jurkat cells.

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  WB analysis of Jurkat using 22803-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22803-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22803-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22803-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 22803-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22803-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22803-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22803-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22803-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 22803-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 22803-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22803-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 22803-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22803-1-AP (IGF2BP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22803-1-AP

  IP result of anti-IGF2BP1 (IP:22803-1-AP, 4ug; Detection:22803-1-AP 1:1000) with HEK-293 cells lysate 1480ug.

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  IHC staining of human lung cancer using 22803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 22803-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22803-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A375 using 22803-1-AP

  Immunofluorescent analysis of A375 cells using 22803-1-AP (IGF2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A375 using 22803-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using IGF2BP1 antibody (22803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

IGF2BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22803-1-AP

Publications(95)

Reviews (3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

IMP 1, IGF-II mRNA-binding protein 1, IGF2BP 1, IGF2 mRNA-binding protein 1, CRD-BP

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, mouse kidney tissue, A375 cells, rat kidney tissue, HepG2 cells, HuH-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 24 publications below
• WB: See 76 publications below
• IHC: See 11 publications below
• IF: See 13 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below
• RIP: See 18 publications below

Product Information

22803-1-AP targets IGF2BP1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGF2BP1 fusion protein Ag18859
• Full Name: insulin-like growth factor 2 mRNA binding protein 1
• Calculated Molecular Weight: 577 aa, 63 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC156957
• Gene Symbol: IGF2BP1
• Gene ID (NCBI): 10642
• RRID: AB_2879173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZI8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF2BP1, also named as CRDBP, VICKZ1, ZBP1, is a 577 amino acid protein, which belongs to the RRM IMP/VICKZ family. IGF2BP1 can form homodimers and heterodimers with IGF2BP1 and IGF2BP3. IGF2BP1 is mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus and also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta. IGF2BP1 is Expressed in various cancers, including testis and lung cancers, as well as kidney, prostate and trachea cancers. IGF2BP1 as RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. The calcualted molecular weight of IGF2BP1 is 63 kDa, but modified IGF2BP1 is about 65-70 kDa.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun ; KD Validated
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza ; KD Validated
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
mouse	IHC,IF,WB	ACS Cent Sci; Allosteric Regulation of IGF2BP1 as a Novel Strategy for the Activation of Tumor Immune Microenvironment; Authors - Yang Liu
human	WB	Mol Cell; m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3; Authors - Ting Shan ; KD Validated
human,mouse	WB	Adv Sci (Weinh); SREBP1c-Mediated Transcriptional Repression of YME1L1 Contributes to Acute Kidney Injury by Inducing Mitochondrial Dysfunction in Tubular Epithelial Cells; Authors - Wang Xin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

Worked very well to detect IGF2BP1 at around 70 kDa by WB in HeLa cells (with an overnight incubation at 4°C, 1/1000 dilution)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa cells

FH

tao (Verified Customer) (12-14-2022)

Immunofluorescence seems to work, but signal can be detected in the nucleus

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50

FH

Parisa (Verified Customer) (06-13-2022)

I used the antibody and it worked very well, It was sensitive and could detect the faint band that I expected in my IP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293