Validation Data Gallery
|Positive WB detected in
|Positive IHC detected in
|human liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:3000
|IHC : 1:50-1:500
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
18500-1-AP targets IGFBP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
|Host / Isotype
|Rabbit / IgG
|IGFBP4 fusion protein Ag13347
|insulin-like growth factor binding protein 4
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401). IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.
The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain.
Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.
SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells
High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis.
FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization