IGFBP4 Polyclonal antibody

IGFBP4 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18500-1-AP

Synonyms

BP 4, HT29 IGFBP, IBP 4, IBP4, IGF binding protein 4, IGFBP 4, IGFBP4

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Validation Data Gallery

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  WB analysis of C6 using 18500-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 18500-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of C6 using 18500-1-AP

  Immunofluorescent analysis of C6 cells using 18500-1-AP (IGFBP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells
• Positive IHC detected in: human liver tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 18500-1-AP is IGFBP4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Cited Reactivity: human, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGFBP4 fusion protein Ag13347
• Full Name: insulin-like growth factor binding protein 4
• Calculated molecular weight: 28 kDa
• Observed molecular weight: 28-40 kDa
• GenBank accession number: BC016041
• Gene symbol: IGFBP4
• Gene ID (NCBI): 3487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.

Publications

Species	Application	Title
human	IF	Sci Adv; The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain.; Authors - Cynthia Hajal
human	WB	Theranostics; Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.; Authors - Mengmeng Qin
human	WB	BMC Biol; SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells; Authors - Yang Yang
human	IHC	Front Oncol; High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis.; Authors - Wenwen Guo
chicken	WB	Cell Signal; PMID: 28734787; Authors - Jie Yang