IGFBP4 Polyclonal antibody
IGFBP4 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 18500-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | C6 cells |
Positive IHC detected in | human liver tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | C6 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 18500-1-AP is IGFBP4 Fusion Protein expressed in E. coli.
Tested Reactivity | human, rat |
Cited Reactivity | human, chicken |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IGFBP4 fusion protein Ag13347 |
Full Name | insulin-like growth factor binding protein 4 |
Calculated molecular weight | 28 kDa |
Observed molecular weight | 28-40 kDa |
GenBank accession number | BC016041 |
Gene symbol | IGFBP4 |
Gene ID (NCBI) | 3487 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for IGFBP4 antibody 18500-1-AP | Download protocol |
IHC protocol for IGFBP4 antibody 18500-1-AP | Download protocol |
IF protocol for IGFBP4 antibody 18500-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Theranostics Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions. | ||
BMC Biol SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells | ||
Front Oncol High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis. | ||