|Positive WB detected in||C6 cells|
|Positive IHC detected in||human liver tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C6 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18500-1-AP is IGFBP4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat|
|Cited Reactivity||chicken, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IGFBP4 fusion protein Ag13347|
|Full Name||insulin-like growth factor binding protein 4|
|Calculated molecular weight||28 kDa|
|Observed molecular weight||28-40 kDa|
|GenBank accession number||BC016041|
|Gene ID (NCBI)||3487|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.
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