IGFBP4 Polyclonal antibody

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  WB analysis of C6 using 18500-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 18500-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 18500-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of C6 using 18500-1-AP

  Immunofluorescent analysis of C6 cells using 18500-1-AP (IGFBP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

IGFBP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18500-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IBP4, IBP 4, HT29 IGFBP, Deb2, BP4

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Tested Applications

• Positive WB detected in: C6 cells
• Positive IHC detected in: human liver tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

18500-1-AP targets IGFBP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGFBP4 fusion protein Ag13347
• Full Name: insulin-like growth factor binding protein 4
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-40 kDa
• GenBank Accession Number: BC016041
• Gene Symbol: IGFBP4
• Gene ID (NCBI): 3487
• RRID: AB_2878547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22692
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401). IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.

Publications

Species	Application	Title
human	IF	Sci Adv; The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain.; Authors - Cynthia Hajal
human	WB	Theranostics; Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.; Authors - Mengmeng Qin
human	WB	BMC Biol; SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells; Authors - Yang Yang
human	IHC	Front Oncol; High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis.; Authors - Wenwen Guo
chicken	WB	Cell Signal; PMID: 28734787; Authors - Jie Yang
human	WB	Oral Dis; FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization; Authors - Xiangrui Ma