IGFBP4 Polyclonal antibody

IGFBP4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18500-1-AP

Synonyms

BP 4, HT29 IGFBP, IBP 4, IBP4, IGF binding protein 4, IGFBP 4, IGFBP4



Tested Applications

Positive WB detected inC6 cells
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

18500-1-AP targets IGFBP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP4 fusion protein Ag13347
Full Name insulin-like growth factor binding protein 4
Calculated molecular weight 28 kDa
Observed molecular weight 28-40 kDa
GenBank accession numberBC016041
Gene symbol IGFBP4
Gene ID (NCBI) 3487
RRIDAB_2878547
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401). IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.

Protocols

Product Specific Protocols
WB protocol for IGFBP4 antibody 18500-1-APDownload protocol
IHC protocol for IGFBP4 antibody 18500-1-APDownload protocol
IF protocol for IGFBP4 antibody 18500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Adv

The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain.

Authors - Cynthia Hajal
humanWB

Theranostics

Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.

Authors - Mengmeng Qin
humanWB

BMC Biol

SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells

Authors - Yang Yang
humanIHC

Front Oncol

High Serpin Family A Member 10 Expression Confers Platinum Sensitivity and Is Associated With Survival Benefit in High-Grade Serous Ovarian Cancer: Based on Quantitative Proteomic Analysis.

Authors - Wenwen Guo
chickenWB

Cell Signal

PMID: 28734787

Authors - Jie Yang
humanWB

Oral Dis

FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization

Authors - Xiangrui Ma