IGFBP5 Polyclonal antibody

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  WB analysis of SKOV-3 using 55205-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 55205-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse embryo using 55205-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 55205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55205-1-AP (IGFBP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55205-1-AP (IGFBP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 55205-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using IGFBP5 antibody (55205-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

IGFBP5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 55205-1-AP

Publications(21)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5

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Tested Applications

• Positive WB detected in: SKOV-3 cells, HepG2 cells, mouse embryo tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Product Information

55205-1-AP targets IGFBP5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: insulin-like growth factor binding protein 5
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: NM_000599
• Gene Symbol: IGFBP5
• Gene ID (NCBI): 3488
• RRID: AB_2736835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24593
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.

Publications

Species	Application	Title
human	IF	Theranostics; Material-induced Senescence (MIS): Fluidity Induces Senescent Type Cell Death of Lung Cancer Cells via Insulin-Like Growth Factor Binding Protein 5.; Authors - Sharmy Saimon Mano
human	WB	Oncogene; Fibulin-3 promotes muscle-invasive bladder cancer.; Authors - A L Han
human	IF	Breast Cancer Res; Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors; Authors - Wei Yang
mouse	WB	Transl Res; PMID: 30392876; Authors - Lin Xihua
human	WB	J Proteomics; Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.; Authors - Sun-Xia Chen
rat	WB	Am J Physiol Heart Circ Physiol; Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.; Authors - Guangrui Lai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Margarita (Verified Customer) (06-06-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: CWR22Rv1 cells

IGFBP5 Antibody Immunofluorescence validation (1:100 dilution) in CWR22Rv1 cells (Cat no:55205-1-AP)