IGFBP5 Polyclonal antibody

IGFBP5 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 55205-1-AP

Synonyms

IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5



Tested Applications

Positive WB detected inSKOV-3 cells, mouse embryo tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

55205-1-AP targets IGFBP5 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name insulin-like growth factor binding protein 5
Calculated molecular weight 31 kDa
Observed molecular weight 33 kDa
GenBank accession numberNM_000599
Gene symbol IGFBP5
Gene ID (NCBI) 3488
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.

Protocols

Product Specific Protocols
WB protocol for IGFBP5 antibody 55205-1-APDownload protocol
IHC protocol for IGFBP5 antibody 55205-1-APDownload protocol
IF protocol for IGFBP5 antibody 55205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma.

Authors - Mayinuer Maitituoheti
WB

Vet Comp Oncol

Significant association of serum autoantibodies to TYMS, HAPLN1 and IGFBP5 with early stage canine malignant mammary tumours.

Authors - Shih-Chieh Chang
humanWB

Biomed Res Int

Insulin-Like Growth Factor Binding Protein 5-A Probable Target of Kidney Renal Papillary Renal Cell Carcinoma.

Authors - Shuqiang Wang
humanWB

Prenat Diagn

Differences in IGF axis-related proteins in amniotic fluid of trisomy 21 and trisomy 18 using a multiple reaction monitoring (MRM) approach.

Authors - Guangrui Lai
ratWB

Am J Physiol Heart Circ Physiol

Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.

Authors - Guangrui Lai
humanWB

J Proteomics

Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.

Authors - Sun-Xia Chen