Tested Applications
| Positive WB detected in | SKOV-3 cells, mouse embryo tissue |
| Positive IHC detected in | human kidney tissue, mouse ovary tissue, rat ovary tissue, human placenta tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55205-1-AP targets IGFBP5 in WB, IHC, IF-P, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | insulin-like growth factor binding protein 5 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | NM_000599 |
| Gene Symbol | IGFBP5 |
| Gene ID (NCBI) | 3488 |
| RRID | AB_2736835 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P24593 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IGFBP5 antibody 55205-1-AP | Download protocol |
| IHC protocol for IGFBP5 antibody 55205-1-AP | Download protocol |
| WB protocol for IGFBP5 antibody 55205-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IGFBP5 Polyclonal antibody (catalog number 55205-1-AP) has 44 citations. These appear across high-impact journals including Cell, Nature Neuroscience, Nature Communications, Cell Stem Cell, Oncogene, Theranostics, Cell Reports, Cell Death & Disease, and Frontiers in Immunology, among others. Research fields span oncology (lung, bladder, breast, colorectal, glioma, melanoma, ovarian cancer), metabolic and renal disease (diabetic nephropathy, insulin resistance), cardiovascular disorders (atherosclerosis, heart failure), neurology (stroke, Alzheimer's), fibrosis, and immunology. Applications include WB, IF, IHC, ELISA, and ChIP.
| Species | Application | Title |
|---|---|---|
Cell A blood-brain barrier-like vascular gate limits immunotherapy efficacy in neuroendocrine cancers. | ||
Cell Stem Cell SPTEdU-seq enables parallel optics-free newborn cell tracking and spatial total transcriptional dynamics in intact microenvironments. | ||
Nat Neurosci Spinal cord Tau pathology induces tactile deficits and cognitive impairment in Alzheimer's disease via dysregulation of CCK neurons | ||
Nat Commun Ultra-precision deconvolution of spatial transcriptomics decodes immune heterogeneity and fate-defining programs in tissues. | ||
Nat Commun Recurrence in the chemotherapy regimen of bladder carcinoma originates from quiescent epidermoid-like cells. | ||
Theranostics Material-induced Senescence (MIS): Fluidity Induces Senescent Type Cell Death of Lung Cancer Cells via Insulin-Like Growth Factor Binding Protein 5. |
Reviews
What customers say
The product received a single 5-star review. The reviewer used it for immunofluorescence on CWR22Rv1 cells at a 1:100 dilution and included an image of the results, indicating strong performance and satisfaction with the staining outcome. No written comments were provided beyond the rating and experimental details.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Margarita (Verified Customer) (06-06-2023) |
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