|Positive WB detected in||SKOV-3 cells, mouse embryo tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SKOV-3 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
55205-1-AP targets IGFBP5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||insulin-like growth factor binding protein 5|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||NM_000599|
|Gene ID (NCBI)||3488|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.
Material-induced Senescence (MIS): Fluidity Induces Senescent Type Cell Death of Lung Cancer Cells via Insulin-Like Growth Factor Binding Protein 5.
Fibulin-3 promotes muscle-invasive bladder cancer.
Breast Cancer Res
Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors
Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.
Am J Physiol Heart Circ Physiol
Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Margarita (Verified Customer) (06-06-2023)