IGFBP5 Polyclonal antibody
IGFBP5 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 55205-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SKOV-3 cells, mouse embryo tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 8 publications below |
| IF | See 1 publications below |
Product Information
55205-1-AP targets IGFBP5 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | insulin-like growth factor binding protein 5 |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 33 kDa |
| GenBank accession number | NM_000599 |
| Gene symbol | IGFBP5 |
| Gene ID (NCBI) | 3488 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IGFBP5 antibody 55205-1-AP | Download protocol |
| IHC protocol for IGFBP5 antibody 55205-1-AP | Download protocol |
| IF protocol for IGFBP5 antibody 55205-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma. | ||
Vet Comp Oncol Significant association of serum autoantibodies to TYMS, HAPLN1 and IGFBP5 with early stage canine malignant mammary tumours. | ||
Biomed Res Int Insulin-Like Growth Factor Binding Protein 5-A Probable Target of Kidney Renal Papillary Renal Cell Carcinoma. | ||
Prenat Diagn Differences in IGF axis-related proteins in amniotic fluid of trisomy 21 and trisomy 18 using a multiple reaction monitoring (MRM) approach. | ||
Am J Physiol Heart Circ Physiol Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5. | ||
J Proteomics Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation. |
