IGFBP5 Polyclonal antibody

IGFBP5 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 55205-1-AP

Synonyms

IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5

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Validation Data Gallery

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  WB analysis of SKOV-3 using 55205-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 55205-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 55205-1-AP (IGFBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 55205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55205-1-AP (IGFBP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55205-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55205-1-AP (IGFBP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SKOV-3 using 55205-1-AP

  Immunofluorescent analysis of SKOV-3 cells using 55205-1-AP (IGFBP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, mouse embryo tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

55205-1-AP targets IGFBP5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: insulin-like growth factor binding protein 5
• Calculated molecular weight: 31 kDa
• Observed molecular weight: 33 kDa
• GenBank accession number: NM_000599
• Gene symbol: IGFBP5
• Gene ID (NCBI): 3488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.

Publications

Species	Application	Title
human	IF	Theranostics; Material-induced Senescence (MIS): Fluidity Induces Senescent Type Cell Death of Lung Cancer Cells via Insulin-Like Growth Factor Binding Protein 5.; Authors - Sharmy Saimon Mano
human	WB	Oncogene; Fibulin-3 promotes muscle-invasive bladder cancer.; Authors - A L Han
mouse	WB	Transl Res; PMID: 30392876; Authors - Lin Xihua
human	WB	J Proteomics; Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.; Authors - Sun-Xia Chen
rat	WB	Am J Physiol Heart Circ Physiol; Homocysteine downregulates cardiac homeobox transcription factor NKX2.5 via IGFBP5.; Authors - Guangrui Lai
human	WB	Prenat Diagn; Differences in IGF axis-related proteins in amniotic fluid of trisomy 21 and trisomy 18 using a multiple reaction monitoring (MRM) approach.; Authors - Guangrui Lai

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