IGFN1 Polyclonal antibody

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  WB analysis using 25095-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25095-1-AP (IGFN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

IGFN1 Polyclonal Antibody for WB, ELISA

Cat No. 25095-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KYIP1, EEF1A2BP1

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25095-1-AP targets IGFN1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18731

  Product name: Recombinant human IGFN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 42-385 aa of BC131529

  Sequence: IYGERRDATRSSTSRYKPGTGSFSKDAQGPMGHFSQGLADMEVQPGEAATLSCTLTSDLGPGTWFKDGVKLTTQDGVIFKQDGLVHSLFITHVQGTQAGRYTFVAGDQQSEATLTVQDSPTIAPDVTEKLREPLVVKAGKPVIVKIPFQSHLPIQAAWRKDGAEVVGSSDREAQVDLGDGYTRLCLPSAGRKDCGQYSVTLRSEGGSVQAELTLQVIDKPDPPQGPMEVQDCHRAGVCLRWRPPRDNGGRTVECYVVERRQAGRSTWLKVGEAPADSTTFTDAHVEPGRKYTFRVRAVTSEGAGEALESEEILVAPEALPKAPSAPAILSASSQGITLTWTAPR

• Full Name: immunoglobulin-like and fibronectin type III domain containing 1
• Calculated Molecular Weight: 1251 aa, 138 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC131529
• Gene Symbol: IGFN1
• Gene ID (NCBI): 91156
• RRID: AB_3669462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86VF2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Immunoglobulin-like and fibronectin type III domain-containing protein 1 (IGFN1), also known as EEF1A2-binding protein 1 (EEF1A2BP1) or KY-interacting protein 1 (KYIP1), presents structural similarity to sarcomeric proteins belonging to the intracellular Ig superfamily, including myosin-binding protein C fast and slow-type skeletal muscle isoforms (PMID: 18756455). IGFN1 along with KY and FLNC is the part of a Z-band associated protein complex likely providing structural support to the skeletal muscle (PMID: 20206623). IGFN1 gene has been associated with some cancers (PMID: 28027327; 30335789; 35456975). At least five isoforms of IGFN1 produced by alternative splicing have been identified (PMID: 20206623).