human IgG heavy chain Polyclonal antibody

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  WB analysis of human plasma using 16402-1-AP

  human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 16402-1-AP (human IgG heavy chain Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16402-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16402-1-AP (human IgG heavy chain antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 16402-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16402-1-AP (human IgG heavy chain antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 16402-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16402-1-AP (human IgG heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

human IgG heavy chain Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16402-1-AP

Publications(17)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, CoIP, RIP, ELISA

Synonyms

Human IgG4, IgG Fc, IGHG4, IGHG, IgG4

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Tested Applications

• Positive WB detected in: human plasma tissue, Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 3 publications below

Product Information

16402-1-AP targets human IgG heavy chain in WB, IHC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9519

  Product name: Recombinant human IGHG4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 124-473 aa of BC025985

  Sequence: AAGHLVMGFGAHWGQGKLVSVSPASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPSCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGK

• Full Name: immunoglobulin heavy constant gamma 4 (G4m marker)
• Calculated Molecular Weight: 473 aa, 52 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC025985
• Gene Symbol: IGHG4
• Gene ID (NCBI): 3503
• RRID: AB_2878254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01861
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGHG4 has some fragments with MW 36 kDa (http://www.uniprot.org/uniprot/P01861) and 52 kDa (http://www.ncbi.nlm.nih.gov/protein/CAE45900). This antibody detects all the Heavy chain of human immunoglobulin gamma (IgG), IgG1, IgG2, IgG3, IgG4 included.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IHC	Nat Commun; Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia.; Authors - Franc Llorens
	IHC	Neuropathol Appl Neurobiol; Plasma total prion protein as a potential biomarker for neurodegenerative dementia: diagnostic accuracy in the spectrum of prion diseases.; Authors - Franc Llorens
	CoIP	Mol Oncol; Secretogranin II impairs tumor growth and angiogenesis by promoting degradation of hypoxia-inducible factor-1α in colorectal cancer.; Authors - Chao Fang
	WB,IP	Cancer Gene Ther; miR-451a was selectively sorted into exosomes and promoted the progression of esophageal squamous cell carcinoma through CAB39; Authors - Lu Wang
	RIP	J Dent Sci; Circ_0004491 stimulates guanine nucleotide-binding protein alpha subunit to inhibit the malignant progression of oral squamous cell carcinoma by sponging miR-2278; Authors - Tao Cheng
	WB	Oncol Rep; Upregulation of IGF1 by tumor-associated macrophages promotes the proliferation and migration of epithelial ovarian cancer cells.; Authors - Li Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Pratheepa (Verified Customer) (06-11-2021)

The antibody works excellent. But the specificity is not restricted to human cells. It picks up mouse cell line also.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Adipose stem cell, Mouse microglial cell

human IgG heavy chain Antibody Immunofluorescence validation (1:200 dilution) in Adipose stem cell, Mouse microglial cell (Cat no:16402-1-AP)