human IgG heavy chain Polyclonal antibody

human IgG heavy chain Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16402-1-AP

Synonyms

human IgG heavy chain, IgG Fc, IGHG, IGHG4, Immunoglobulin heavy chain gamma



Tested Applications

Positive WB detected inhuman plasma tissue, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Human IgG4 Ready-To-Use IHC Kit. Human IgG4 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16402-1-AP targets human IgG heavy chain in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen human IgG heavy chain fusion protein Ag9519
Full Name immunoglobulin heavy constant gamma 4 (G4m marker)
Calculated molecular weight 473 aa, 52 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC025985
Gene symbol IGHG4
Gene ID (NCBI) 3503
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGHG4 has some fragments with MW 36 kDa (http://www.uniprot.org/uniprot/P01861) and 52 kDa (http://www.ncbi.nlm.nih.gov/protein/CAE45900). This antibody detects all the Heavy chain of human immunoglobulin gamma (IgG), IgG1, IgG2, IgG3, IgG4 included.

Protocols

Product Specific Protocols
WB protocol for human IgG heavy chain antibody 16402-1-APDownload protocol
IHC protocol for human IgG heavy chain antibody 16402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Ther Med

PCSK9 regulates apoptosis in human lung adenocarcinoma A549 cells via endoplasmic reticulum stress and mitochondrial signaling pathways.

Authors - Xiaohui Xu
humanWB

Oncol Lett

Oridonin induces apoptosis in HGC-27 cells by activating the JNK signaling pathway.

Authors - Dong-Lin Ren
humanIP

J Physiol Biochem

Transgelin-2 interacts with CD44 to regulate Notch1 signaling pathway and participates in colorectal cancer proliferation and migration.

Authors - Rong Ding
RIP

Front Genet

A Circ-0007022/miR-338-3p/Neuropilin-1 Axis Reduces the Radiosensitivity of Esophageal Squamous Cell Carcinoma by Activating Epithelial-To-Mesenchymal Transition and PI3K/AKT Pathway.

Authors - Junpeng Zhang

Int J Mol Sci

Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling.

Authors - Zhifeng Wu
WB

Oncol Rep

Upregulation of IGF1 by tumor-associated macrophages promotes the proliferation and migration of epithelial ovarian cancer cells.

Authors - Li Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Pratheepa (Verified Customer) (06-11-2021)

The antibody works excellent. But the specificity is not restricted to human cells. It picks up mouse cell line also.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Adipose stem cell, Mouse microglial cell
human IgG heavy chain Antibody Immunofluorescence validation (1:200 dilution) in Adipose stem cell, Mouse microglial cell (Cat no:16402-1-AP)