Tested Applications
| Positive WB detected in | Ramos cells, human plasma, mouse spleen tissue, mouse plasma, rat plasma |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13688-1-AP targets IGJ in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4559 Product name: Recombinant human IGJ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-159 aa of BC038982 Sequence: MKNHLLFWGVLAVFIKAVHVKAQEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD Predict reactive species |
| Full Name | immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides |
| Calculated Molecular Weight | 158 aa, 18 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC038982 |
| Gene Symbol | IGJ |
| Gene ID (NCBI) | 3512 |
| RRID | AB_2877973 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01591 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGJ antibody 13688-1-AP | Download protocol |
| IP protocol for IGJ antibody 13688-1-AP | Download protocol |
| WB protocol for IGJ antibody 13688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IGJ polyclonal antibody (Cat# 13688-1-AP) has a total of 5 citations. These appear in Phytomedicine, International Immunopharmacology, Journal of Biotechnology, Plant Molecular Biology, and Oncology Letters. The associated research fields include intestinal immunology and sIgA secretion, gastric cancer immune infiltration modeling, recombinant secretory IgA production, plant-derived IgA engineering, and JNK signaling pathway-mediated apoptosis.
| Species | Application | Title |
|---|---|---|
Phytomedicine The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides. | ||
Int Immunopharmacol Integrative analysis-based identification and validation of a prognostic immune cell infiltration-based model for patients with advanced gastric cancer. | ||
J Biotechnol Efficient production of recombinant secretory IgA against Clostridium difficile toxins in CHO-K1 cells. | ||
Plant Mol Biol Plant-derived secretory component gives protease-resistance to Shiga toxin 1-specific dimeric IgA. | ||
Oncol Lett Oridonin induces apoptosis in HGC-27 cells by activating the JNK signaling pathway. |
Reviews
What customers say
One reviewer (Leon B.) rated this antibody 5/5 stars, using it at a 1:1000 dilution on a mouse B cell line for Western Blot. The reviewer reported that the antibody "worked as intended" and produced a clean single band, indicating strong specificity and performance under their experimental conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Leon (Verified Customer) (01-30-2026) | Worked as intended, clean single band.
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