IGJ Polyclonal antibody

IGJ Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13688-1-AP

Synonyms

IGCJ, IGJ, Immunoglobulin J chain, JCH



Tested Applications

Positive WB detected inhuman plasma tissue, mouse spleen tissue, human plasma
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13688-1-AP targets IGJ in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGJ fusion protein Ag4559
Full Name immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
Calculated molecular weight 158 aa, 18 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC038982
Gene symbol IGJ
Gene ID (NCBI) 3512
RRIDAB_2877973
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.

Protocols

Product Specific Protocols
WB protocol for IGJ antibody 13688-1-APDownload protocol
IHC protocol for IGJ antibody 13688-1-APDownload protocol
IP protocol for IGJ antibody 13688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Phytomedicine

The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides.

Authors - Gaofeng Cai
humanIHC

Int Immunopharmacol

Integrative analysis-based identification and validation of a prognostic immune cell infiltration-based model for patients with advanced gastric cancer.

Authors - Siwei Pan
hamsterWB

J Biotechnol

Efficient production of recombinant secretory IgA against Clostridium difficile toxins in CHO-K1 cells.

Authors - Venugopal Bhaskara
WB

Plant Mol Biol

Plant-derived secretory component gives protease-resistance to Shiga toxin 1-specific dimeric IgA.

Authors - Katsuhiro Nakanishi