IGJ Polyclonal antibody

IGJ Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13688-1-AP

Synonyms

Joining chain of multimeric IgA and IgM, JCHAIN, JCH, Immunoglobulin J chain, IGCJ

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Validation Data Gallery

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  WB analysis of human plasma using 13688-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 13688-1-AP (IGJ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 13688-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 13688-1-AP (IGJ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13688-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13688-1-AP (IGJ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 13688-1-AP

  IP result of anti-IGJ (IP:13688-1-AP, 4ug; Detection:13688-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human tonsillitis using 13688-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13688-1-AP (IGJ antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13688-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13688-1-AP (IGJ antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human plasma tissue, mouse spleen tissue, human plasma
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

13688-1-AP targets IGJ in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGJ fusion protein Ag4559
• Full Name: immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
• Calculated Molecular Weight: 158 aa, 18 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC038982
• Gene Symbol: IGJ
• Gene ID (NCBI): 3512
• RRID: AB_2877973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides.; Authors - Gaofeng Cai
human	IHC	Int Immunopharmacol; Integrative analysis-based identification and validation of a prognostic immune cell infiltration-based model for patients with advanced gastric cancer.; Authors - Siwei Pan
hamster	WB	J Biotechnol; Efficient production of recombinant secretory IgA against Clostridium difficile toxins in CHO-K1 cells.; Authors - Venugopal Bhaskara
	WB	Plant Mol Biol; Plant-derived secretory component gives protease-resistance to Shiga toxin 1-specific dimeric IgA.; Authors - Katsuhiro Nakanishi