Anti-Human IGSF8/CD316 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of human peripheral whole blood using 98530-2-RR (same clone as 98530-2-PBS)

  1x10^6 human peripheral whole blood cells were surface stained with 0.25 ug Anti-Human IGSF8/CD316 Rabbit RecAb (98530-2-RR, Clone: 243019D8) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98530-2-PBS in a different storage buffer formulation.

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  FC experiment of Transfected CHO using 98530-2-RR (same clone as 98530-2-PBS)

  1x10^6 CHO cells or IGSF8/CD316-transfected CHO cells were surface stained with 0.25 ug Anti-Human IGSF8/CD316 Rabbit RecAb (98530-2-RR, Clone: 243019D8) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 98530-2-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected CHO using 98530-2-RR (same clone as 98530-2-PBS)

  1x10^6 CHO cells or IGSF8/CD316 transfected CHO cells were surface stained with 0.25 ug Anti-Human IGSF8/CD316 Rabbit RecAb (98530-2-RR, Clone: 243019D8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 98530-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98530-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98530-2-RR against Human IGSF8/CD316 were performed. The affinity constant is 0.166 nM.

IGSF8/CD316 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98530-2-PBS

Clone No.243019D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

IgSF8, CD316, CD81 partner 3, CD81P3, EWI 2

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Product Information

98530-2-PBS targets IGSF8/CD316 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2664

  Product name: recombinant human IGSF8 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 28-579 aa of NM_052868.6

  Sequence: REVLVPEGPLYRVAGTAVSISCNVTGYEGPAQQNFEWFLYRPEAPDTALGIVSTKDTQFSYAVFKSRVVAGEVQVQRLQGDAVVLKIARLQAQDAGIYECHTPSTDTRYLGSYSGKVELRVLPDVLQVSAAPPGPRGRQAPTSPPRMTVHEGQELALGCLARTSTQKHTHLAVSFGRSVPEAPVGRSTLQEVVGIRSDLAVEAGAPYAERLAAGELRLGKEGTDRYRMVVGGAQAGDAGTYHCTAAEWIQDPDGSWAQIAEKRAVLAHVDVQTLSSQLAVTVGPGERRIGPGEPLELLCNVSGALPPAGRHAAYSVGWEMAPAGAPGPGRLVAQLDTEGVGSLGPGYEGRHIAMEKVASRTYRLRLEAARPGDAGTYRCLAKAYVRGSGTRLREAASARSRPLPVHVREEGVVLEAVAWLAGGTVYRGETASLLCNISVRGGPPGLRLAASWWVERPEDGELSSVPAQLVGGVGQDGVAELGVRPGGGPVSVELVGPRSHRLRLHSLGPEDEGVYHCAPSAWVQHADYSWYQAGSARSGPVTVYPYMHALDT

• Full Name: immunoglobulin superfamily, member 8
• Calculated Molecular Weight: 65 kDa
• GenBank Accession Number: NM_052868.6
• Gene Symbol: IGSF8
• Gene ID (NCBI): 93185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q969P0-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IGSF8 is also known as CD316, PGRL (PG regulatory-like protein), KAI/CD82-associated surface molecule, and CD81-binding partner 3 (CD81P3). IGSF8 contains four immunoglobulin domains, a transmembrane region, and a short cytoplasmic tail that does not bear any signature motif for signal transduction. IGSF8 is abundantly expressed in olfactory sensory neuron (OSN) axons and their developing synapses. (PMID: 17785435, PMID: 22687584)