IKBKB Polyclonal antibody

• Featured Product
• KD/KO Validated

IKBKB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

30

Cat no : 15649-1-AP

Synonyms

I kappa B kinase 2, I kappa B kinase beta, IKBKB, IKK B, IKK beta, IKK2, IKKB, IκB, NFKBIKB

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Validation Data Gallery

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  WB analysis of Jurkat using 15649-1-AP

  WB result of IKBKB antibody (15649-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-IKBKB transfected Jurkat cells.

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  WB analysis of Jurkat using 15649-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, K-562 cells
• Positive IHC detected in: human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 24 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

15649-1-AP targets IKBKB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IKBKB fusion protein Ag8191
• Full Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Calculated molecular weight: 756aa,81 kDa; 256aa,29 kDa
• Observed molecular weight: 80 kDa, 86 kDa, 87 and 29 kDa
• GenBank accession number: BC006231
• Gene symbol: IKBKB
• Gene ID (NCBI): 3551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IKBKB, also named as IKKB, IKK2, NFKBIKB and IKK-B, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB is a Serine kinase that plays an essential role in the NF-kappa-B signaling pathway. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. In addition to the NF-kappa-B inhibitors, IKBKB phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. It also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, IKBKB acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. This antibody can identify 4 isoform of IKBKB with the molecular weight of 80, 86, 87 and 29 kDa.

Publications

Species	Application	Title
mouse	WB	Int Immunopharmacol; Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide/d-galactosamine induced acute liver failure in mice.; Authors - Yutong He
human	WB	Molecules; Effects of Betaine on LPS-Stimulated Activation of Microglial M1/M2 Phenotypes by Suppressing TLR4/NF-κB Pathways in N9 Cells.; Authors - Hui Shi
human	IHC	Cell Mol Immunol; ACPAs promote IL-1β production in rheumatoid arthritis by activating the NLRP3 inflammasome.; Authors - Xiwen Dong
mouse	WB	J Agric Food Chem; Comparison of the Hepatoprotective Effects of the Three Main Stilbenes from Mulberry Twigs.; Authors - Ya-Nan Jia
human	WB	Arch Oral Biol; A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.; Authors - Shichen Sun
mouse	IP	Front Genet; Suppression of Conditional TDP-43 Transgene Expression Differentially Affects Early Cognitive and Social Phenotypes in TDP-43 Mice.; Authors - Pablo R Silva