IKBKB Polyclonal antibody

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  WB analysis of Jurkat using 15649-1-AP

  WB result of IKBKB antibody (15649-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-IKBKB transfected Jurkat cells.

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  WB analysis of Jurkat using 15649-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15649-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15649-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15649-1-AP (IKBKB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15649-1-AP

  IP result of anti-IKBKB (IP:15649-1-AP, 4ug; Detection:15649-1-AP 1:3000) with Jurkat cells lysate 1400 ug.

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  IHC staining of human liver cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15649-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15649-1-AP (IKBKB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

IKBKB Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15649-1-AP

Publications(107)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

IKKB, EC:2.7.11.1, EC:2.7.11.10, I kappa B kinase 2, I kappa B kinase beta

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, HepG2 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 99 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

15649-1-AP targets IKBKB in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IKBKB fusion protein Ag8191
• Full Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Calculated Molecular Weight: 756aa,81 kDa; 256aa,29 kDa
• Observed Molecular Weight: 80 kDa, 86 kDa, 87 and 29 kDa
• GenBank Accession Number: BC006231
• Gene Symbol: IKBKB
• Gene ID (NCBI): 3551
• RRID: AB_2122307
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14920
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKBKB, also named as IKKB, IKK2, NFKBIKB and IKK-B, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and I-kappa-B kinase subfamily. IKBKB is a Serine kinase that plays an essential role in the NF-kappa-B signaling pathway. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. In addition to the NF-kappa-B inhibitors, IKBKB phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. It also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, IKBKB acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. This antibody can identify 4 isoform of IKBKB with the molecular weight of 80, 86, 87 and 29 kDa.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice; Authors - Xiaoxue Jiang
mouse	WB	Adv Sci (Weinh); Sirtuin 5-Mediated Desuccinylation of ALDH2 Alleviates Mitochondrial Oxidative Stress Following Acetaminophen-Induced Acute Liver Injury; Authors - Qiwen Yu
mouse	WB	Drug Des Devel Ther; Sichen Formula Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via Blocking the TLR4 Signaling Pathways; Authors - Li-Shan Yan
human	IF	Cell Death Discov; tRNA-derived fragment TRF365 regulates the metabolism of anterior cruciate ligament cells by targeting IKBKB.; Authors - Dianbo Long
rat	WB	Redox Biol; Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro.; Authors - Dejuan Li
		Br J Pharmacol; PMID: 28963856; Authors - Xiao Han

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Eugenia (Verified Customer) (03-16-2022)

Antibody worked well with specific band although a couple of non-specific bands also appeared.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: A549 lung cancer cell line